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Literature summary for 2.7.7.13 extracted from

  • Sacchetti, S.; Bartolucci, S.; Rossi, M.; Cannio, R.
    Identification of a GDP-mannose pyrophosphorylase gene from Sulfolobus solfataricus (2004), Gene, 332, 149-157.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis, expression analysis and regulation of the expression, functional expression as GST-tagged enzyme in Escherichia coli strain BL21 under conditions suitable to reduce the formation of inclusion bodies using the vector pTgroE of the yeast Vig9 protein, overview Saccharolobus solfataricus
fused to glutathione S-transferase, expression in Escherichia coli, TOP10F' Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40520
-
calculated from amino acid sequence Saccharolobus solfataricus
42000
-
SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Saccharolobus solfataricus
-
diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate Saccharolobus solfataricus regulation of the enzyme expression, overview diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate Saccharolobus solfataricus P2
-
diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate Saccharolobus solfataricus P2 regulation of the enzyme expression, overview diphosphate + GDP-mannose
-
r

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity by glutathione sepharose affinity chromatography Saccharolobus solfataricus

Renatured (Commentary)

Renatured (Comment) Organism
to homogeneity Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + diphosphate
-
Saccharolobus solfataricus GTP + D-mannose 1-phosphate
-
r
GDP-mannose + diphosphate
-
Saccharolobus solfataricus P2 GTP + D-mannose 1-phosphate
-
r
GTP + alpha-D-mannose 1-phosphate
-
Saccharolobus solfataricus diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate regulation of the enzyme expression, overview Saccharolobus solfataricus diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate the enzyme shows high specificity for synthesizing GDP-mannose, D-glucose 1-phosphate instead of alpha-D-mannose 1-phosphate is a poor substrate Saccharolobus solfataricus diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate
-
Saccharolobus solfataricus P2 diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate regulation of the enzyme expression, overview Saccharolobus solfataricus P2 diphosphate + GDP-mannose
-
r
GTP + alpha-D-mannose 1-phosphate the enzyme shows high specificity for synthesizing GDP-mannose, D-glucose 1-phosphate instead of alpha-D-mannose 1-phosphate is a poor substrate Saccharolobus solfataricus P2 diphosphate + GDP-mannose
-
r
GTP + D-mannose 1-phosphate
-
Saccharolobus solfataricus diphosphate + GDP-mannose
-
r
GTP + D-mannose 1-phosphate
-
Saccharolobus solfataricus P2 diphosphate + GDP-mannose
-
r

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
GDP-Man pyrophosphorylase
-
Saccharolobus solfataricus
GDP-mannose pyrophosphorylase
-
Saccharolobus solfataricus
GMPP
-
Saccharolobus solfataricus
GTP-alpha-D-Man-1-P guanylyltransferase
-
Saccharolobus solfataricus
guanidine diphosphomannose pyrophoyphorylase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
forward reaction, assay at Saccharolobus solfataricus
60
-
reverse reaction, assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
reverse reaction, assay at Saccharolobus solfataricus
7.6
-
forward reaction, assay at Saccharolobus solfataricus