Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis, expression analysis and regulation of the expression, functional expression as GST-tagged enzyme in Escherichia coli strain BL21 under conditions suitable to reduce the formation of inclusion bodies using the vector pTgroE of the yeast Vig9 protein, overview | Saccharolobus solfataricus |
fused to glutathione S-transferase, expression in Escherichia coli, TOP10F' | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40520 | - |
calculated from amino acid sequence | Saccharolobus solfataricus |
42000 | - |
SDS-PAGE | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Saccharolobus solfataricus | - |
diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | Saccharolobus solfataricus | regulation of the enzyme expression, overview | diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | Saccharolobus solfataricus P2 | - |
diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | Saccharolobus solfataricus P2 | regulation of the enzyme expression, overview | diphosphate + GDP-mannose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity by glutathione sepharose affinity chromatography | Saccharolobus solfataricus |
Renatured (Comment) | Organism |
---|---|
to homogeneity | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + diphosphate | - |
Saccharolobus solfataricus | GTP + D-mannose 1-phosphate | - |
r | |
GDP-mannose + diphosphate | - |
Saccharolobus solfataricus P2 | GTP + D-mannose 1-phosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Saccharolobus solfataricus | diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | regulation of the enzyme expression, overview | Saccharolobus solfataricus | diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | the enzyme shows high specificity for synthesizing GDP-mannose, D-glucose 1-phosphate instead of alpha-D-mannose 1-phosphate is a poor substrate | Saccharolobus solfataricus | diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Saccharolobus solfataricus P2 | diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | regulation of the enzyme expression, overview | Saccharolobus solfataricus P2 | diphosphate + GDP-mannose | - |
r | |
GTP + alpha-D-mannose 1-phosphate | the enzyme shows high specificity for synthesizing GDP-mannose, D-glucose 1-phosphate instead of alpha-D-mannose 1-phosphate is a poor substrate | Saccharolobus solfataricus P2 | diphosphate + GDP-mannose | - |
r | |
GTP + D-mannose 1-phosphate | - |
Saccharolobus solfataricus | diphosphate + GDP-mannose | - |
r | |
GTP + D-mannose 1-phosphate | - |
Saccharolobus solfataricus P2 | diphosphate + GDP-mannose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
GDP-Man pyrophosphorylase | - |
Saccharolobus solfataricus |
GDP-mannose pyrophosphorylase | - |
Saccharolobus solfataricus |
GMPP | - |
Saccharolobus solfataricus |
GTP-alpha-D-Man-1-P guanylyltransferase | - |
Saccharolobus solfataricus |
guanidine diphosphomannose pyrophoyphorylase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
forward reaction, assay at | Saccharolobus solfataricus |
60 | - |
reverse reaction, assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reverse reaction, assay at | Saccharolobus solfataricus |
7.6 | - |
forward reaction, assay at | Saccharolobus solfataricus |