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2.6.1.5: tyrosine transaminase

This is an abbreviated version!
For detailed information about tyrosine transaminase, go to the full flat file.

Word Map on EC 2.6.1.5

Reaction

L-tyrosine
+
2-oxoglutarate
=
4-hydroxyphenylpyruvate
 +
L-glutamate

Synonyms

aminotransferase, tyrosine, At5g36160, At5g53970, beta-phenylalanine aminotransferase, glutamic phenylpyruvic aminotransferase, glutamic-hydroxyphenylpyruvic transaminase, L-phenylalanine 2-oxoglutarate aminotransferase, L-tyrosine aminotransferase, L-tyrosine-2-oxoglutarate aminotransferase, L-tyrosine: 2 oxoglutarate aminotransferase, L-tyrosine: 2-oxoglutarate aminotransferase, More, mTAT, phenylalanine aminotransferase, phenylalanine transaminase, phenylalanine-alpha-ketoglutarate transaminase, phenylpyruvate transaminase, phenylpyruvic acid transaminase, PPY-AT, Sar2028, TAT, TAT1, TAT2, TAT3, TATase, TATc, Tyr aminotransferase, TyrAT, tyrosine aminotransferase, tyrosine-2-ketoglutarate aminotransferase, tyrosine-2-oxoglutarate aminotransferase, tyrosine-alpha-ketoglutarate aminotransferase, tyrosine-alpha-ketoglutarate transaminase, VpAT

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.5 tyrosine transaminase

Purification

Purification on EC 2.6.1.5 - tyrosine transaminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 enzyme forms isolated using pyridoxamine-affinity column chromatography
-
4 enzyme forms separated by isoelectric focusing and hydroxyapatite chromatography, one of them is probably identical to mitochondrial L-aspartate aminotransferase EC 2.6.1.1
-
high yield procedure using a thermal inactivation of the lysosomal converting factor that generates two additional, lower molecular weight forms
-
His tag affinity column chromatography
HisTrap column chromatography and Sephadex G50 gel filtration
isoenzymes TAT-I and TAT-II
-
native and one of the modified forms
-
Ni-NTA column chromatography
nickel Sepharose 6 fast flow chromatography, HiPrep 26/10 desalting column chromatography, and S-75 gel filtration
A0A163U8E7
NTA-resin column chromatography
-
partial
partial purification of enzyme expressed in Saccharomyces cerevisiae and Escherichia coli using affinity chromatography
-
partial purification of two isoenzymes, using several chromatographic steps
-
partial, using ammonium sulfate precipitation and several chromatographic steps
-
partial, using chromatography on DEAE-cellulose and gel filtration
-
partial, using gel filtration
-
partial, using pyridoxamine-affinity column cheomatography
-
partial, using Sephadex chromatography and isoelectrofocusing
-
separation of 4 enzyme forms: I, II, III, IV, using hydroxyapatite chromatography
-
three isoenzymes TAT-1, TAT-2 and TAT-3, isolated using anion-exchange chromatography and electrofocusing
-
using affinity, Source-Q and gel-filtration chromatography
-
using Ni-NTA chromatography