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Literature summary for 2.6.1.5 extracted from

  • Dietrich, J.B.; Lorber, B.; Kern, D.
    Expression of mammalian tyrosine aminotransferase in Saccharomyces cerevisiae and Escherichia coli. Purification to homogeneity and characterization of the enzyme overproduced in the bacteria (1991), Eur. J. Biochem., 201, 399-407.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of rat liver enzyme in Saccharomyces cerevisiae and Escherichia coli Rattus norvegicus

General Stability

General Stability Organism
pyridoxal 5'-phosphate protects against thermal inactivation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Canaline
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
2-oxoglutarate pH 7.2, 37°C Rattus norvegicus
3.4
-
L-tyrosine pH 7.2, 37°C Rattus norvegicus
60
-
tryptophan pH 7.2, 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Rattus norvegicus
110000
-
recombinant expressed in Escherichia coli, ultracentrifugation Rattus norvegicus
150000
-
recombinant expressed in Escherichia coli, native gradient PAGE Rattus norvegicus
160000
-
recombinant expressed in Escherichia coli, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of enzyme expressed in Saccharomyces cerevisiae and Escherichia coli using affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus
416
-
enzyme expressed in Escherichia coli Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 2-oxoglutarate
-
Rattus norvegicus phenylpyruvate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate
-
Rattus norvegicus 4-hydroxyphenylpyruvate + L-glutamate
-
?
tryptophan + 2-oxoglutarate
-
Rattus norvegicus 3-indole-2-oxopropanoate + L-glutamate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
pyridoxal 5'-phosphate protects against thermal inactivation Rattus norvegicus
additional information
-
the apoenzyme is thermally unstable: t1/2 of 2 min at 55°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
recombinant enzyme expressed in Escherichia coli Rattus norvegicus