Cloned (Comment) | Organism |
---|---|
expression of rat liver enzyme in Saccharomyces cerevisiae and Escherichia coli | Rattus norvegicus |
General Stability | Organism |
---|---|
pyridoxal 5'-phosphate protects against thermal inactivation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Canaline | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
2-oxoglutarate | pH 7.2, 37°C | Rattus norvegicus | |
3.4 | - |
L-tyrosine | pH 7.2, 37°C | Rattus norvegicus | |
60 | - |
tryptophan | pH 7.2, 37°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Rattus norvegicus |
110000 | - |
recombinant expressed in Escherichia coli, ultracentrifugation | Rattus norvegicus |
150000 | - |
recombinant expressed in Escherichia coli, native gradient PAGE | Rattus norvegicus |
160000 | - |
recombinant expressed in Escherichia coli, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of enzyme expressed in Saccharomyces cerevisiae and Escherichia coli using affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
416 | - |
enzyme expressed in Escherichia coli | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2-oxoglutarate | - |
Rattus norvegicus | phenylpyruvate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Rattus norvegicus | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
tryptophan + 2-oxoglutarate | - |
Rattus norvegicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
pyridoxal 5'-phosphate protects against thermal inactivation | Rattus norvegicus |
additional information | - |
the apoenzyme is thermally unstable: t1/2 of 2 min at 55°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
recombinant enzyme expressed in Escherichia coli | Rattus norvegicus |