Crystallization (Comment) | Organism |
---|---|
spontaneous crystallization when enzyme concentration is about 10 mg protein per ml | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-Methyl-L-aspartate | with L-tyrosine and 2-oxoglutarate or oxaloacetate as substrates | Rattus norvegicus | |
beta-Methyl-L-aspartate | with L-tyrosine and 2-oxoglutarate or oxaloacetate as substrates | Rattus norvegicus | |
D-Aspartate | weak inhibition | Rattus norvegicus | |
L-aspartate | with L-tyrosine and 2-oxoglutarate or oxaloacetate as substrates | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
amino acid composition and gel filtration | Rattus norvegicus |
114000 | - |
sedimentation analysis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using Sephadex chromatography and isoelectrofocusing | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.155 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-chlorophenyl)alanine + 2-oxoglutarate | 17% of the activity with L-tyrosine | Rattus norvegicus | 3-(3-chlorophenyl)-2-oxopropanoate + L-glutamate | - |
? | |
3,4-dihydroxyphenylalanine + 2-oxoglutarate | 85% of the activity than with L-tyrosine | Rattus norvegicus | 3-(3,4-dihydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
? | |
3-iodotyrosine + 2-oxoglutarate | 84% of the activity than with L-tyrosine | Rattus norvegicus | 3-(4-hydroxy-3-iodophenyl)-2-oxopropanoate + L-glutamate | - |
? | |
3-methoxytyrosine + 2-oxoglutarate | 22% of the activity with L-tyrosine | Rattus norvegicus | 3-(3-methoxy-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
? | |
4-hydroxyphenylpyruvate + L-aspartate | 35% of the activity with L-glutamate | Rattus norvegicus | L-tyrosine + oxaloacetate | - |
r | |
L-asparagine + 2-oxoglutarate | 15% of the activity with L-tyrosine | Rattus norvegicus | 2-oxosuccinamate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | higher activity than with L-tyrosine | Rattus norvegicus | oxaloacetate + L-glutamate | - |
? | |
L-cysteine + 2-oxoglutarate | higher activity than with L-tyrosine | Rattus norvegicus | 3-mercapto-2-oxopropanoate + L-glutamate | - |
? | |
L-ethionine + 2-oxoglutarate | 17% of the activity than with L-tyrosine | Rattus norvegicus | 4-ethylsulfanyl-2-oxobutanoate + L-glutamate | - |
? | |
L-methionine + 2-oxoglutarate | 20% of the activity than with L-tyrosine | Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | higher rate than with L-tyrosine | Rattus norvegicus | phenylpyruvate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Rattus norvegicus | 4-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-tyrosine + oxaloacetate | - |
Rattus norvegicus | 4-hydroxyphenylpyruvate + L-aspartate | - |
r | |
tryptophan + 2-oxoglutarate | 62% of the activity than with L-tyrosine | Rattus norvegicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the mitochondrial enzyme may be identical with EC 2.6.1.1 and with EC 2.6.1.57 | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |