Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)RIL cells | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania major | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49600 | - |
calculated from amino acid sequence | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + 2-oxoglutarate | - |
Leishmania major | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Leishmania major | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
additional information | leishmanial TAT is deprived of ALAT activity and exhibits undetectable activity towards leucine, glutamine and cysteine, leishmanial TAT reveals undetectable activity when other 2-oxoacids such as 2-oxoglutarate, glyoxylate, 2-oxoisovalerate and 2-oxoisocaproate are assayed at a concentration up to 10 mM, the leishmanial enzyme displays almost identical preference for 2-oxo-4-methylthiobutyrate, pyruvate and 2-oxobutyrate | Leishmania major | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TAT | - |
Leishmania major |
tyrosine aminotransferase | - |
Leishmania major |