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Literature summary for 2.6.1.5 extracted from

  • Seetharamappa, J.; Oke, M.; Liu, H.; McMahon, S.A.; Johnson, K.A.; Carter, L.; Dorward, M.; Zawadzki, M.; Overton, I.M.; van Niekirk, C.A.; Graham, S.; Botting, C.H.; Taylor, G.L.; White, M.F.; Barton, G.J.; Coote, P.J.; Naismith, J.H.
    Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5-phosphate-dependent aminotransferase (2007), Acta Crystallogr. Sect. F, F63, 452-456.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 23.6% (w/v) PEG 4000, 0.1 M HEPES, pH 8.0 Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48170
-
X-ray crystallography Staphylococcus aureus
50000
-
SDS-PAGE Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A163U8E7
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel Sepharose 6 fast flow chromatography, HiPrep 26/10 desalting column chromatography, and S-75 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 2-oxoglutarate
-
Staphylococcus aureus 4-hydroxyphenylpyruvate + L-glutamate
-
?

Subunits

Subunits Comment Organism
dimer S-75 gel filtration Staphylococcus aureus

Synonyms

Synonyms Comment Organism
Sar2028
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent Staphylococcus aureus

pI Value

Organism Comment pI Value Maximum pI Value
Staphylococcus aureus 2D-PAGE
-
5.25