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2.5.1.15: dihydropteroate synthase

This is an abbreviated version!
For detailed information about dihydropteroate synthase, go to the full flat file.

Word Map on EC 2.5.1.15

Reaction

(7,8-dihydropterin-6-yl)methyl diphosphate
+
4-Aminobenzoate
=
diphosphate
 +
7,8-dihydropteroate

Synonyms

6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydropterin-pyrophosphokinase-dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydropteroate synthase, 7,8-dihydropteroate synthase, 7,8-dihydropteroate synthetase, 7,8-dihydropteroic acid synthetase, BCG3672c, DHP synthase, DHPS, DHPS-HPPK, DHPS/DHPR, dihydroneopterin aldolase-dihydropterin pyrophosphokinase-dihydropteroate synthase, dihydropteroate diphosphorylase, dihydropteroate synthase, dihydropteroate synthase/hydroxymethyldihydropterin pyrophosphokinase, dihydropteroate synthetase, dihydropteroic synthetase, FolP, folP1, folP2, HPPK-DHPS, hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase, MbDHPS, MtDHPS, Mycobacterium tuberculosis dihydropteroate synthase, PfDHPS, pfPPK-DHPS, PPPK-DHPS, putative dihydropteroate synthase ortholog, pvdhps, PvHPPK-DHPS, PVX_123230, Rv1207, synthase, dihydropteroate

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.15 dihydropteroate synthase

Purification

Purification on EC 2.5.1.15 - dihydropteroate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anion exchange and affinity column
-
enzyme from recombinant clones in Sephacryl S-300 HR, enzyme isolated form Escherichia coli, several chromatographic columns
-
gel filtration and ion-exchange chromatography
-
gel filtration on Sephadex G-100
-
His-tagged recombinant protein by fast-performance liquid chromatography
mycobacterial enzymes, from DEAE-Sepharose and other chromatography steps
Ni-NTA agarose column chromatography, Q-Sepharose column chromatography, phenyl FF 16/10 column chromatography, and Superdex S-200 gel filtration
Ni-Sepharose column chromatography
overexpression of the N-terminal His6 fusion protein in Escherichia coli, coexpression with chaperone genes to increase solubility
precipitation from bacterial lysate by ammonium sufate (50% saturation), resuspension in Tris/HCl pH 8, Resource Q ion-exchange chromatography (elution with NaCl gradient), dialysis, Mono Q ion-exchange chromatography (elution with NaCl gradient), for crystallisation followed by Superdex 200 gel-filtration chromatography
precipitation with ammonium sulfate followed by column chromatography
Q-Sepharose column
-
recombinant bifunctional fusion protein encoding dihydropterin pyrophosphokinase and dihyropteroate synthase domains of the trifunctional enzyme dihydroneopterin aldolase-dihydropterin pyrophosphokinase-dihydropteroate synthase
recombinant enzyme, affinity column
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by nickel affinity chromatography and gel filtration
recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase
-
recombinant, hexa-His tagged enzyme from bacterial lysate by nickel-nitrilotriacetic acid agarose, elution with 200 mM imidazole, stored at -80°C in 10% glycerol or further purified on by Superdex 200 16/60 gel filtration chromatography
recombinant, hexa-His tagged enzyme from bacterial lysate by nickel-nitrilotriacetic acid agarose, elution with 200 mM imidazole, stored at -80°C in 10% glycerol or further purified on by Superdex 200 GL 10/300 gel filtration chromatography
Superdex-200 HR and other chromatographic columns
-