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2.5.1.15: dihydropteroate synthase

This is an abbreviated version!
For detailed information about dihydropteroate synthase, go to the full flat file.

Word Map on EC 2.5.1.15

Reaction

(7,8-dihydropterin-6-yl)methyl diphosphate
+
4-Aminobenzoate
=
diphosphate
 +
7,8-dihydropteroate

Synonyms

6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydropterin-pyrophosphokinase-dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydropteroate synthase, 7,8-dihydropteroate synthase, 7,8-dihydropteroate synthetase, 7,8-dihydropteroic acid synthetase, BCG3672c, DHP synthase, DHPS, DHPS-HPPK, DHPS/DHPR, dihydroneopterin aldolase-dihydropterin pyrophosphokinase-dihydropteroate synthase, dihydropteroate diphosphorylase, dihydropteroate synthase, dihydropteroate synthase/hydroxymethyldihydropterin pyrophosphokinase, dihydropteroate synthetase, dihydropteroic synthetase, FolP, folP1, folP2, HPPK-DHPS, hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase, MbDHPS, MtDHPS, Mycobacterium tuberculosis dihydropteroate synthase, PfDHPS, pfPPK-DHPS, PPPK-DHPS, putative dihydropteroate synthase ortholog, pvdhps, PvHPPK-DHPS, PVX_123230, Rv1207, synthase, dihydropteroate

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.15 dihydropteroate synthase

Cloned

Cloned on EC 2.5.1.15 - dihydropteroate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a single polypeptide, Fas protein, expressed in cultured Spodoptera frugiperda SF9 insect cells
-
cDNA encoding dihydropterin pyrophosphokinase and dihyropteroate synthase domains of the trifunctional enzyme dihydroneopterin aldolase-dihydropterin pyrophosphokinase-dihydropteroate synthase is cloned. This bi-functional enzyme is expressed as a His6 fusion protein in Escherichia coli
cloning of a sulfonamide resistant strain
-
crystal structure of the liganded enzyme and of the enzyme in complex with 6-hydroxymethyl-pterine-diphosphate, 6-hydroxymethyl-pterine-monophosphate, 6-methylamino-5-nitroisocytosine, or pteroic acid. Hanging drop vapor diffusion method, the crystals are space group P6(2)22 with cell dimensions a = b = 97.4 A, c = 263.6 A
engineering dihydropteroate synthase for efficient expression on M13 phage
-
expressed in Escherichia coli
-
expressed in Escherichia coli Rosetta pLysS cells
expressed in Escherichia coli Rosetta(DE3)pLysS cells
expressed under the control of the T5 promoter in a DHPS-deficient Escherichia coli strain
-
expression in different Escherichia coli strains
-
expression in dihydroxypteroate synthase deficient strain of Escherichia coli
expression in Escherichia coli
expression in Escherichia coli as N-terminal His6 fusion
expression in Escherichia coli BL21
-
expression in Escherichia coli C600 and in Escherichia coli folP knockout mutant
expression in Escherichia coli Rosetta(DE3)pLysS
-
expression in Saccharomyces cerevisiae Fol1 knockout Y26466
expression of recombinant enzyme in Escherichia coli
-
from genomic DNA (strain H37Rv) in pET-21a for expression with C-terminal hexa-His tag in Escherichia coli BL21 (DE3)
from genomic DNA (strain H37Rv) in pQE60 for expression with C-terminal hexa-His tag in Escherichia coli M15
from Streptococcus pneumoniae for expression in Escherichia coli XL2-Blue
gene encoding bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase, cloned and expressed in Escherichia coli
-
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)