Application | Comment | Organism |
---|---|---|
drug development | cannot bypass DHPS activity during inhibition of the functional ortholog MtDHPS (folP1) as treatment of tuberculosis | Mycobacterium tuberculosis |
drug development | target in treatment of tuberculosis | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
from genomic DNA (strain H37Rv) in pET-21a for expression with C-terminal hexa-His tag in Escherichia coli BL21 (DE3) | Mycobacterium tuberculosis |
from genomic DNA (strain H37Rv) in pQE60 for expression with C-terminal hexa-His tag in Escherichia coli M15 | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
PDB: 2VP8, conforms classical triosephosphate isomerase (TIM) barrel arrangement, 6-hydroxymethyl-7,8-dihydropterin-diphosphate-binding pocket occupied by the histidine 220 side chain compared to leucine 180 in the functional ortholog MtDHPS (PDB: 1EYE), crystals: space group P3(1)21, 2 molecules per asymmetric unit, noncrystallographic dimer in the crystal structure, unit cell parameters: a, b: 80.73, c: 215.94, hanging drop vapour method: 25% ethylene glycole + protein solution (16 mg/ml), 2 days, 18°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
recombinant, hexa-His tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
33000 | - |
recombinant, hexa-His tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
34070 | - |
deduced from primary structure | Mycobacterium tuberculosis |
75000 | - |
recombinant hexa-His tagged enzyme, gel filtration chromatography | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNC9 | - |
- |
Mycobacterium tuberculosis | P9WND1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNC9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WND1 | - |
- |
Mycobacterium tuberculosis variant bovis | A0A0H3MCB3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant, hexa-His tagged enzyme from bacterial lysate by nickel-nitrilotriacetic acid agarose, elution with 200 mM imidazole, stored at -80°C in 10% glycerol or further purified on by Superdex 200 16/60 gel filtration chromatography | Mycobacterium tuberculosis |
recombinant, hexa-His tagged enzyme from bacterial lysate by nickel-nitrilotriacetic acid agarose, elution with 200 mM imidazole, stored at -80°C in 10% glycerol or further purified on by Superdex 200 GL 10/300 gel filtration chromatography | Mycobacterium tuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | logarithmic growing bacteria, Western blotting | Mycobacterium tuberculosis variant bovis | - |
cell suspension culture | qiescent bacteria, lower expression level under anaerobic conditions compared to nutrient starvation, Western blotting | Mycobacterium tuberculosis variant bovis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
+/-3, assumed inactive because 100times less than folP1 gene product | Mycobacterium tuberculosis |
0.577 | - |
+/-12, pH 9 | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | binding of 6-hydroxymethyl-7,8-dihydropterin-diphosphate with KD: 117 microM, revealed by fluorescence spectroscopy | Mycobacterium tuberculosis | ? | - |
? | |
additional information | binding of 6-hydroxymethyl-7,8-dihydropterin-diphosphate with KD: 4.6 microM, revealed by fluorescence spectroscopy | Mycobacterium tuberculosis | ? | - |
? | |
additional information | binding of dapsone (p-aminobenzoate substrate analog) with KD: 168 microM, in absence of 6-hydroxymethyl-7,8-dihydropterin-diphosphate, , revealed by fluorescence spectroscopy | Mycobacterium tuberculosis | ? | - |
? | |
additional information | binding of dapsone (p-aminobenzoate substrate analog) with KD: 96 microM, in absence of 6-hydroxymethyl-7,8-dihydropterin-diphosphate, revealed by fluorescence spectroscopy | Mycobacterium tuberculosis | ? | - |
? | |
additional information | Rv1207 lacks dihydropteroate synthase activity in presence of 10 microM p-aminobenzoate and 10 microM 6-hydroxymethyl-7,8-dihydropterin-diphosphate, 5 mM MgCl2, 1 mM dithiothreitol, 37°C, pH 7-9.5 | Mycobacterium tuberculosis | ? | - |
? | |
additional information | binding of 6-hydroxymethyl-7,8-dihydropterin-diphosphate with KD: 117 microM, revealed by fluorescence spectroscopy | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | binding of dapsone (p-aminobenzoate substrate analog) with KD: 96 microM, in absence of 6-hydroxymethyl-7,8-dihydropterin-diphosphate, revealed by fluorescence spectroscopy | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | Rv1207 lacks dihydropteroate synthase activity in presence of 10 microM p-aminobenzoate and 10 microM 6-hydroxymethyl-7,8-dihydropterin-diphosphate, 5 mM MgCl2, 1 mM dithiothreitol, 37°C, pH 7-9.5 | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | binding of 6-hydroxymethyl-7,8-dihydropterin-diphosphate with KD: 4.6 microM, revealed by fluorescence spectroscopy | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | binding of dapsone (p-aminobenzoate substrate analog) with KD: 168 microM, in absence of 6-hydroxymethyl-7,8-dihydropterin-diphosphate, , revealed by fluorescence spectroscopy | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
p-aminobenzoate + 6-hydroxymethyl-7,8-dihydropterin-diphosphate | 5 mM MgCl2, 1 mM dithiothreitol, 37°C, pH 7-9.5 | Mycobacterium tuberculosis | diphosphate + 7,8-dihydropteroate | - |
? | |
p-aminobenzoate + 6-hydroxymethyl-7,8-dihydropterin-diphosphate | 5 mM MgCl2, 1 mM dithiothreitol, 37°C, pH 7-9.5 | Mycobacterium tuberculosis H37Rv | diphosphate + 7,8-dihydropteroate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34070, gel filtration chromatography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
BCG3672c | - |
Mycobacterium tuberculosis variant bovis |
DHPS | - |
Mycobacterium tuberculosis |
folP1 | - |
Mycobacterium tuberculosis |
folP2 | - |
Mycobacterium tuberculosis |
MbDHPS | - |
Mycobacterium tuberculosis variant bovis |
MtDHPS | - |
Mycobacterium tuberculosis |
Mycobacterium tuberculosis dihydropteroate synthase | - |
Mycobacterium tuberculosis |
putative dihydropteroate synthase ortholog | - |
Mycobacterium tuberculosis |
Rv1207 | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
according to specific activity | Mycobacterium tuberculosis |