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6.5.1.2: DNA ligase (NAD+)

This is an abbreviated version!
For detailed information about DNA ligase (NAD+), go to the full flat file.

Word Map on EC 6.5.1.2

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
 +
AMP

Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.2 DNA ligase (NAD+)

Temperature Stability

Temperature Stability on EC 6.5.1.2 - DNA ligase (NAD+)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
the half-life of heat inactivation at 100°C is about 3 min
24
-
stable for 1 week
37
-
stable for 1 week
4
-
activity decreases remarkably below 4°C
65
-
2 days, 50% loss of activity
70
-
wild-type enzyme is stable for 40 min, about 20% loss of activity after 180 min. Mutant enzyme DELTA582-667 loses 20% of its activity after 80 min and 90% of its activity after 170 min
72
-
2 h, stable below, destabilized above
90
-
wild-type enzyme is stable for 20 min, about 50% loss of activity after 100 min. Mutant enzyme DELTA582-667 loses 30% of its initial activity after 40 min, completely loses activity after 80 min
additional information