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6.5.1.2: DNA ligase (NAD+)

This is an abbreviated version!
For detailed information about DNA ligase (NAD+), go to the full flat file.

Word Map on EC 6.5.1.2

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
 +
AMP

Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.2 DNA ligase (NAD+)

Activating Compound

Activating Compound on EC 6.5.1.2 - DNA ligase (NAD+)

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
with 30 mM (NH4)2SO4, the activity is maximal
bovine serum albumin
0.025 mg/ml, stimulating
-
dithiothreitol
10 mM, stimulating
Macromolecular solutes
-
blunt-end ligation in presence of high concentrations of macromolecular solutes, inactive in absence, e.g. polyethylene glycol 6000, bovine plasma albumin, or Ficoll 70
-
NaCl
highest activity at 200 mM
NH4Cl
highest activity at 200 mM
polyamines
-
blunt-end ligation are linear oligomers. Optimal concentration: caldopentamine, 0.05 mM, thermine, 0.1-0.2 mM, spermine, 0.2 mM, thermospermine, 0.4 mM, sperminediol, 0.75 mM
-
Polyethylene glycol
-
in presence of high molecular weight polyethylene glycol 20000, 6000 or 1000, 8-28%, the enzyme catalyzes blunt-end intermolecular joining to yield linear oligomers, but no circular DNA forms. In presence of low molecular polyethylene glycols 400, 200, 8-80%, or the monomer ethylene glycol, 16-80%, the circular forms formed by intramolecular ligation are also detected
potassium glutamate
highest activity at 350 mM
Rad51
-
Rad51 enhances Escherichia coli DNA ligase-catalyzed DNA ligation to produce multimers in an ADP-dependent manner
-
RecA
-
when incubated with linear double stranded DNA with 3' four-nucleotide overhangs and ATP, RecA greatly accelerates the end-joining to form multimers of the double stranded DNA catalyzed by NAD-dependent Escherichia coli DNA ligase
-
additional information
-