Cloned (Comment) | Organism |
---|---|
deletion mutants, expression in Escherichia coli | Thermus filiformis |
Crystallization (Comment) | Organism |
---|---|
- |
Thermus filiformis |
Protein Variants | Comment | Organism |
---|---|---|
DELTA582-667 | lower thermal stability than wild-type enzyme | Thermus filiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates wild-type enzyme and mutant enzyme DELTA582-667 | Thermus filiformis | |
Mg2+ | best metal-activator of wild-type enzyme and mutant enzyme DELTA582-667 | Thermus filiformis | |
Mn2+ | activates wild-type enzyme and mutant enzyme DELTA582-667 | Thermus filiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus filiformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutants expressed in Escherichia coli | Thermus filiformis |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent DNA ligase | - |
Thermus filiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
mutant enzyme DELTA582-667 and wild-type enzyme, activity of mutant enzyme DELTA582-667 is 50% of wild-type activity | Thermus filiformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 50% of maximal activity of wild-type enzyme and of mutant enzyme DELTA582-667, 80°C: about 50% of maximal activity of wild-type enzyme and mutant enzyme DELTA582-667 | Thermus filiformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
wild-type enzyme is stable for 40 min, about 20% loss of activity after 180 min. Mutant enzyme DELTA582-667 loses 20% of its activity after 80 min and 90% of its activity after 170 min | Thermus filiformis |
90 | - |
wild-type enzyme is stable for 20 min, about 50% loss of activity after 100 min. Mutant enzyme DELTA582-667 loses 30% of its initial activity after 40 min, completely loses activity after 80 min | Thermus filiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
wild-type enzyme | Thermus filiformis |
8.5 | - |
mutant enzyme DELTA582-667 | Thermus filiformis |