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Literature summary for 6.5.1.2 extracted from

  • Jeon, H.J.; Shin, H.J.; Choi, J.J.; Hoe, H.S.; Kim, H.K.; Suh, S.W.; Kwon, S.T.
    Mutational analyses of the thermostable NAD+-dependent DNA ligase from Thermus filiformis (2004), FEMS Microbiol. Lett., 237, 111-118.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
deletion mutants, expression in Escherichia coli Thermus filiformis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Thermus filiformis

Protein Variants

Protein Variants Comment Organism
DELTA582-667 lower thermal stability than wild-type enzyme Thermus filiformis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates wild-type enzyme and mutant enzyme DELTA582-667 Thermus filiformis
Mg2+ best metal-activator of wild-type enzyme and mutant enzyme DELTA582-667 Thermus filiformis
Mn2+ activates wild-type enzyme and mutant enzyme DELTA582-667 Thermus filiformis

Organism

Organism UniProt Comment Textmining
Thermus filiformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutants expressed in Escherichia coli Thermus filiformis

Synonyms

Synonyms Comment Organism
NAD+-dependent DNA ligase
-
Thermus filiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
mutant enzyme DELTA582-667 and wild-type enzyme, activity of mutant enzyme DELTA582-667 is 50% of wild-type activity Thermus filiformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: about 50% of maximal activity of wild-type enzyme and of mutant enzyme DELTA582-667, 80°C: about 50% of maximal activity of wild-type enzyme and mutant enzyme DELTA582-667 Thermus filiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
wild-type enzyme is stable for 40 min, about 20% loss of activity after 180 min. Mutant enzyme DELTA582-667 loses 20% of its activity after 80 min and 90% of its activity after 170 min Thermus filiformis
90
-
wild-type enzyme is stable for 20 min, about 50% loss of activity after 100 min. Mutant enzyme DELTA582-667 loses 30% of its initial activity after 40 min, completely loses activity after 80 min Thermus filiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
wild-type enzyme Thermus filiformis
8.5
-
mutant enzyme DELTA582-667 Thermus filiformis