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6.5.1.2: DNA ligase (NAD+)

This is an abbreviated version!
For detailed information about DNA ligase (NAD+), go to the full flat file.

Word Map on EC 6.5.1.2

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
+
AMP

Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.2 DNA ligase (NAD+)

Molecular Weight

Molecular Weight on EC 6.5.1.2 - DNA ligase (NAD+)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
SDS-PAGE, C-terminal fragment
30040
-
ESI-mass spectrometry, C-terminal fragment
35790
-
ESI-mass spectrometry, N-terminal fragment
36000
-
SDS-PAGE, N-terminal fragment
62000
-
SDS-PAGE, recombinant enzyme
70000
-
x * 70000, SDS-PAGE
72000
-
SDS-PAGE
72130
-
calculated from amino acid sequence
73610
calculated from amino acid sequence
74000
-
1 * 74000, SDS-PAGE of denatured and reduced enzyme
74260
Q-TOF-MS-mass spectrometry, non-adenylated form
74590
Q-TOF-mass spectrometry, adenylated form
75000
-
SDS-PAGE, recombinant protein
75080
calculated from amino acid sequence
75290
-
mass spectrometry
75940
calculated from DNA-sequence
76000
-
SDS-PAGE, recombinant protein
77000
-
sedimentation equilibrium ultracentrifugation
78000
-
x * 78000, SDS-PAGE
78500
-
1 * 78500, SDS-PAGE
79487
x * 79487, calculation from nucleotide sequence
80000
-
x * 80000, SDS-PAGE, DNA-ligase-adenylate intermediate
80400
-
sedimentation equilibrium ultracentrifugation
additional information
-
nucleotide sequence