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6.5.1.2: DNA ligase (NAD+)

This is an abbreviated version!
For detailed information about DNA ligase (NAD+), go to the full flat file.

Word Map on EC 6.5.1.2

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
+
AMP

Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.2 DNA ligase (NAD+)

Purification

Purification on EC 6.5.1.2 - DNA ligase (NAD+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE column chromatography and phosphor-cellulose column chromatography
-
DEAE column chromatographyand phosphor-cellulose column chromatography
-
fragments overexpressed in Escherichia coli
-
heat treatment and immobilized metal affinity column chromatography
-
His-Bind column chromatography
-
His-tag used for purification of recombinant enzymes
-
His10-LigA fusion
-
HisTrap FF column chromatography, hydroxyapatite type I column chromatography, and HiPrep Q column chromatography
large scale
-
MimiLIG is produced in Escherichia coli as a His10-Smt3 fusion and purified from a soluble bacterial lysate by Ni2+-agarose chromatography
-
Ni-agarose column chromatography
Ni-agarose column chromatography, and Superdex 200 gel filtration
-
Ni-NTA agarose column chromatography
Wolbachia sp. subsp. Brugia malayi
recombinant
recombinant enzyme
-
recombinant enzyme from Escherichia coli
recombinant enzymes using His-tag
-
recombinant protein
recombinant protein from Escherichia coli GR501 using His-tag
recombinant protein using His-tag
-
recombinant proteins
-
recombinant proteins from Escherichia coli
-
Sepharose column chromatography and hydroxyapatite column chromatography
-
wild-type and mutants expressed in Escherichia coli
-