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Literature summary for 6.5.1.2 extracted from

  • Le, Y.; Peng, J.; Pei, J.; Li, H.; Duan, Z.; Shao, W.
    Properties of an NAD+-dependent DNA ligase from the hyperthermophile Thermotoga maritima and its application in PCR amplification of long DNA fragments (2010), Enzyme Microb. Technol., 46, 113-117.
No PubMed abstract available
Search for more literature for 6.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme requires a divalent cation like Ca2+, optimal activity at about 20 mM Ca2+ Thermotoga maritima
Mg2+ the enzyme requires a divalent cation like Mg2+, optimal activity at about 20 mM Mg2+ Thermotoga maritima
Mn2+ the enzyme requires a divalent cation like Mn2+, maximal activity at 3 mM Mn2+ Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 x * 78000, SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima ATCC 43589
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment and immobilized metal affinity column chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima Thermotoga maritima ?
-
 ?
additional information purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima Thermotoga maritima ATCC 43589 ?
-
 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hind III digested plasmid Thermotoga maritima AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hind III digested plasmid Thermotoga maritima ATCC 43589 AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
LigA
-
 Thermotoga maritima
NAD+-dependent DNA ligase
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 with Hind III digested plasmid as substrate Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 the purified enzyme has a half-life of over 30 min at 95°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 with Hind III digested plasmid as substrate Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2 8.8 the enzyme retains over 80% of its activity after holding a pH ranging from 7.2 to 8.8 for 1 h at 80°C Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
additional information does not require ATP as cofactor Thermotoga maritima
NAD+ required, optimal activity at 1 mM NAD+ Thermotoga maritima