6.5.1.2: DNA ligase (NAD+)

This is an abbreviated version!
For detailed information about DNA ligase (NAD+), go to the full flat file.

Reaction

Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases

         6.5 Forming phosphoric-ester bonds

             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)

                6.5.1.2 DNA ligase (NAD+)

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Results	in table
16	Activating Compound
48846	AA Sequence
22	Application
1	CAS Registry Number
51	Cloned(Commentary)
120	Cofactor
13	Crystallization (Commentary)
1	Expression
3	General Information
1	General Stability
449	IC50 Value
614	Inhibitors
7	Ki Value [mM]
38	KM Value [mM]
85	Metals/Ions
27	Molecular Weight [Da]
112	Natural Substrates/ Products (Substrates)
877	Organism
56	PDB ID
20	pH Optimum
8	pH Range
1	pH Stability
183	Protein Variants
51	Purification (Commentary)
32	Reaction
1	Reaction Type
193	Reference
5	Specific Activity [micromol/min/mg]
5	Storage Stability
245	Substrates and Products (Substrate)
13	Subunits
249	Synonyms
1	Systematic Name
24	Temperature Optimum [°C]
10	Temperature Range [°C]
16	Temperature Stability [°C]
15	Turnover Number [1/s]

KM Value

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KM Value on EC 6.5.1.2 - DNA ligase (NAD+)

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.14

(dT)10

Thermus thermophilus

-

-

1971

0.000025 - 0.00056

5'-phosphate terminus of deoxyribonucleotides

4 entries

-

0.000025

5'-phosphoryl termini of poly(dA-dT)

Thermus thermophilus

-

-

1971

-

0.0000025

acridinium-ester labelled DNA

Staphylococcus aureus

-

pH 7.4, 21°C

660985

-

0.000028 - 0.000193

deoxyribonucleotide

3 entries

-

0.000136

DNA

Melanoplus sanguinipes

-

pH 7.6, 21°C, C/G matched nick DNA substrate

661248

0.0000185 - 0.076

NAD+

17 entries

0.00008 - 0.105

nicked DNA

10 entries

-

0.000025 - 0.00056

5'-phosphate terminus of deoxyribonucleotides

Salmonella enterica subsp. enterica serovar Typhimurium

-

-

1910

-

0.000025 - 0.00056

5'-phosphate terminus of deoxyribonucleotides

Bacillus subtilis

-

-

1910

-

0.000025 - 0.00056

5'-phosphate terminus of deoxyribonucleotides

Thermus thermophilus

-

-

1910

-

0.000025 - 0.00056

5'-phosphate terminus of deoxyribonucleotides

Escherichia coli

-

-

1910

-

0.000028

deoxyribonucleotide

Streptococcus pneumoniae

-

co-substrate: NAD+, mutant L75F, 25°C, pH 7.5

726920

-

0.000028

deoxyribonucleotide

Streptococcus pneumoniae

-

wild-type, 25°C, pH 7.5

726920

-

0.000193

deoxyribonucleotide

Wolbachia endosymbiont of Brugia malayi

-

pH 8, 25°C

728580

-

0.0000185

NAD+

Thermus thermophilus

-

-

1971

0.00003 - 0.00007

NAD+

Salmonella enterica subsp. enterica serovar Typhimurium

-

-

1910

0.00003 - 0.00007

NAD+

Bacillus subtilis

-

-

1910

0.00003 - 0.00007

NAD+

Thermus thermophilus

-

-

1910

0.00003 - 0.00007

NAD+

Escherichia coli

-

-

1910

0.0013

NAD+

Mycobacterium tuberculosis

-

-

662211

0.00146

NAD+

Mycobacterium tuberculosis

P9WNV1

-

677062

0.0021

NAD+

Streptococcus pneumoniae

-

-

692315

0.00275

NAD+

Staphylococcus aureus

-

pH 7.4, 21°C

660985

0.003

NAD+

Escherichia coli

-

wild type enzyme

653376

0.004

NAD+

Streptococcus pneumoniae

-

wild-type, 25°C, pH 7.5

726920

0.0048

NAD+

Wolbachia endosymbiont of Brugia malayi

-

pH 8, 25°C

728580

0.0058

NAD+

Melanoplus sanguinipes

-

pH 7.6, 21°C

661248

0.009

NAD+

Amsacta moorei entomopoxvirus

-

-

652175

0.026

NAD+

Streptococcus pneumoniae

-

mutant L75F, 25°C, pH 7.5

726920

0.045

NAD+

Escherichia coli

-

E113A mutant enzyme

653376

0.076

NAD+

Escherichia coli

-

Y225A mutant enzyme

653376

0.00008

nicked DNA

Aquifex aeolicus

O66880

pH 7.6, 65°C

653379

-

0.00017

nicked DNA

Pseudoalteromonas haloplanktis

Q9RBF2

4°C, pH 7.8

651073

-

0.00018

nicked DNA

Escherichia coli

-

18°C, pH 7.8

651073

-

0.00024

nicked DNA

Thermus scotoductus

-

45°C, pH 7.8

651073

-

0.0003

nicked DNA

Pseudoalteromonas haloplanktis

Q9RBF2

18°C, pH 7.8

651073

-

0.00047

nicked DNA

Thermus scotoductus

-

60°C, pH 7.8

651073

-

0.00063

nicked DNA

Pseudoalteromonas haloplanktis

Q9RBF2

25°C, pH 7.8

651073

-

0.0007

nicked DNA

Escherichia coli

-

30°C, pH 7.8

651073

-

0.00204

nicked DNA

Escherichia coli

-

45°C, pH 7.8

651073

-

0.105

nicked DNA

Deinococcus radiodurans

-

-

672811

-