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4.2.1.46: dTDP-glucose 4,6-dehydratase

This is an abbreviated version!
For detailed information about dTDP-glucose 4,6-dehydratase, go to the full flat file.

Word Map on EC 4.2.1.46

Reaction

dTDP-alpha-D-glucose
=
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
 +
H2O

Synonyms

DesIV, dTDP-D-glucose 4,6-dehydratase, dTDP-D-glucose oxidoreductase, dTDP-glucose-4,6-dehydratase, dTGD, RmbB, rml-2, RmlB, TDP-D-glucose 4,6-dehydratase, TDP-glucose oxidoreductase, TDPDH, Thymidine diphosphate D-glucose oxidoreductase, thymidine diphosphate-glucose 4,6-dehydratase, Thymidine diphospho-D-glucose 4,6-dehydratase, Thymidine diphosphoglucose oxidoreductase, UDP-D-glucose 4,6-dehydratase, Ugd

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.46 dTDP-glucose 4,6-dehydratase

Cloned

Cloned on EC 4.2.1.46 - dTDP-glucose 4,6-dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) and DH5alpha
expression in Eschericha coli BL21(DE3)
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21(DE3)
gene L780, transcription profiling of UGER, phylogenetic analysis
E3VXL5
gene Z544R, transcription profiling of UGER, phylogenetic analysis
PCR product cloned into pET-32a(+) to construct pDH32 and transformed into Escherichia coli BL21 (DE3) to give pDH32BL for protein expression. PCR product cloned into EcoRI/HindIII of pIBR25 to construct a recombinant vector, pDHC25. The vector pDHC25 subsequently transformed into the rmbB-knockout mutant of Streptomyces peucetius DHNEO by PEG-mediated protoplast transformation