EC Number |
---|
4.2.1.46 | - |
4.2.1.46 | expressed in Escherichia coli |
4.2.1.46 | expressed in Escherichia coli BL21(DE3) and DH5alpha |
4.2.1.46 | expression in Eschericha coli BL21(DE3) |
4.2.1.46 | expression in Escherichia coli |
4.2.1.46 | expression in Escherichia coli BL21 |
4.2.1.46 | expression in Escherichia coli BL21(DE3) |
4.2.1.46 | gene L780, transcription profiling of UGER, phylogenetic analysis |
4.2.1.46 | gene Z544R, transcription profiling of UGER, phylogenetic analysis |
4.2.1.46 | PCR product cloned into pET-32a(+) to construct pDH32 and transformed into Escherichia coli BL21 (DE3) to give pDH32BL for protein expression. PCR product cloned into EcoRI/HindIII of pIBR25 to construct a recombinant vector, pDHC25. The vector pDHC25 subsequently transformed into the rmbB-knockout mutant of Streptomyces peucetius DHNEO by PEG-mediated protoplast transformation |