4.2.1.36: homoaconitate hydratase
This is an abbreviated version!
For detailed information about homoaconitate hydratase, go to the full flat file.
Word Map on EC 4.2.1.36
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4.2.1.36
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alpha-aminoadipate
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homocitrate
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isopropylmalate
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auxotrophy
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homoisocitrate
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l-lysine
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lys4
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jannaschii
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saccharopine
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methanocaldococcus
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synthesis
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medicine
- 4.2.1.36
- alpha-aminoadipate
- homocitrate
- isopropylmalate
-
auxotrophy
- homoisocitrate
- l-lysine
- lys4
- jannaschii
- saccharopine
-
methanocaldococcus
- synthesis
- medicine
Reaction
Synonyms
2-hydroxy-3-carboxyadipate hydro-lyase, cis-Homoaconitase, HACN, HACNMj, Homoaconitase, Homoaconitate hydratase, Hydratase, homoaconitate, leuC, LYS4, lysF, MJ0499, MJ1271
ECTree
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Purification
Purification on EC 4.2.1.36 - homoaconitate hydratase
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HisTrap HP column chromatography and Superdex 200 gel filtration
recombinant wild-type and mutant small and large subunit proteins MJ1271 and MJ1003 from Escherichia coli strain BL21(DE3) by heat treatment at 70°C for 10 min, anion and cation exchange chromatography, adsorption chromatography, and gel filtration
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