4.2.1.36: homoaconitate hydratase

This is an abbreviated version!
For detailed information about homoaconitate hydratase, go to the full flat file.

Reaction

Synonyms

2-hydroxy-3-carboxyadipate hydro-lyase, cis-Homoaconitase, HACN, HACNMj, Homoaconitase, Homoaconitate hydratase, Hydratase, homoaconitate, leuC, LYS4, lysF, MJ0499, MJ1271

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.36 homoaconitate hydratase

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Results	in table
1648	AA Sequence
4	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
3	Expression
8	General Information
2	IC50 Value
13	Inhibitors
1	kcat/KM [mM/s]
16	KM Value [mM]
1	Localization
1	Metals/Ions
2	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
20	Organism
9	Pathway
2	PDB ID
4	pH Optimum
1	pH Range
7	Protein Variants
3	Purification (Commentary)
1	Reaction
2	Reaction Type
20	Reference
1	Source Tissue
28	Substrates and Products (Substrate)
4	Subunits
26	Synonyms
1	Systematic Name
13	Turnover Number [1/s]

General Information

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General Information on EC 4.2.1.36 - homoaconitate hydratase

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

Methanocaldococcus jannaschii

-

the mutations of the small-subunit HACN protein MJ1271 loop-region may reverse the evolution of HACN activity from an ancestral IPMI gene, demonstrating the evolutionary potential for promiscuity in hydro-lyase enzymes. Understanding these specificity determinants enables the functional reannotation of paralogous HACN and isopropylmalate isomerase, IPMI, genes in numerous genome sequences

702368

malfunction

2 entries

metabolism

Cryptococcus neoformans var. grubii

-

the enzyme is involved in the lysine biosynthetic pathway

746978

physiological function

3 entries

additional information

Methanocaldococcus jannaschii

-

the enzymes' stereospecific hydrolyase activity make it an attractive catalyst to produce diastereomers from unsaturated precursors

702368

malfunction

Penicillium chrysogenum

B1P2N4

L2 mutant is deficient in homoaconitase activity since it is complemented by the Aspergillus nidulans lysF gene. Wis 54-1255 gene lys3 complements the L2 mutation. Accumulation of homocitric acid in the L2 strain, resulting from the mutation in the lys3 (homoaconitase) gene, is required for the upregulation of the lysine biosynthetic genes. The mutant Lys3 protein is altered in a region required to bind the [4Fe-4S] cluster

705575

malfunction

Cryptococcus neoformans var. grubii

-

deletion of the LYS4 gene results in lysine auxotrophy. The mutant shows increased sensitivity to oxidative stress, agents that challenge cell wall/membrane integrity, and azole antifungal drugs

746978

physiological function

Aspergillus nidulans

Q92412

lysF gene can complement the Penicillium chrysogenum L2 mutant deficient in homoaconitase activity

705575

physiological function

Methanocaldococcus jannaschii

-

homoaconitase proteins catalyze the isomerization of tricarboxylates with variable chain length gamma-carboxylate groups

702368

physiological function

Cryptococcus neoformans var. grubii

-

Lys4 plays an important role in mitochondrial iron metabolism, and is required for proper mitochondrial function in Cryptococcus neoformans. LYS4 is required for full virulence in Cryptococcus neoformans

746978