4.2.1.36: homoaconitate hydratase

This is an abbreviated version!
For detailed information about homoaconitate hydratase, go to the full flat file.

Reaction

Synonyms

2-hydroxy-3-carboxyadipate hydro-lyase, cis-Homoaconitase, HACN, HACNMj, Homoaconitase, Homoaconitate hydratase, Hydratase, homoaconitate, leuC, LYS4, lysF, MJ0499, MJ1271

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.36 homoaconitate hydratase

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Results	in table
1648	AA Sequence
4	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
3	Expression
8	General Information
2	IC50 Value
13	Inhibitors
1	kcat/KM [mM/s]
16	KM Value [mM]
1	Localization
1	Metals/Ions
2	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
20	Organism
9	Pathway
2	PDB ID
4	pH Optimum
1	pH Range
7	Protein Variants
3	Purification (Commentary)
1	Reaction
2	Reaction Type
20	Reference
1	Source Tissue
28	Substrates and Products (Substrate)
4	Subunits
26	Synonyms
1	Systematic Name
13	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.2.1.36 - homoaconitate hydratase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant protein, micro batch method, 18 mg/ml protein in 20 mM Tris-HCl, pH 8.0, containing 200 mM NaCl and 1 mM DTT, is mixed with reservoir solution, containing 50% w/v PEG 200 and 0.1 M Tris-HCl, pH 4.6, at 0.001 ml each and 22°C, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement method

Methanocaldococcus jannaschii

-

702368

sitting drop vapor diffusion method, small subunit, using 60% (w/v) 2-methyl-2,4-pentanediol and 0.1 M bicine pH 9.7

Methanocaldococcus jannaschii

P81291

726596