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3.5.2.3: dihydroorotase

This is an abbreviated version!
For detailed information about dihydroorotase, go to the full flat file.

Word Map on EC 3.5.2.3

Reaction

(S)-dihydroorotate
+
H2O
=
N-carbamoyl-L-aspartate

Synonyms

amidohydrolase family protein, BcDHOase, CAD, carbamoylaspartic dehydrase, Class I DHOase, DHO, DHOase, dihydroorotase, dihydroorotase domain, dihydroorotate dehydrolase, hDHOase, huDHOase, human DHOase domain, LdDHOase, More, pyrC, type I DHOase, type II DHO, VcDHO, YpDHO

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.3 dihydroorotase

Purification

Purification on EC 3.5.2.3 - dihydroorotase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by chromatography on N-linked gutylamine-agarose and DEAE-Sephacel
-
by Ni-NTA chromatography and Poros Q anion-exchange column
-
chromatography, gel filtration, resource-Q anion exchange column
DEAE-Sephacel column chromatography
dihydroorotase and aspartate transcarbamoylase copurify, partially purified by anion exchange chromatography, gel filtration, hydrophobic interaction chromatography
-
from overexpressing Escherichia coli
-
His-tagged recombinant protein
-
HiTrap Q column chromatography, Hiload Superdex gel filtration, and phenyl-Superose HR column chromatography
-
human stromal cells
-
Mono Q column chromatography
more than 95% purified by PorosHQ column
-
N-linked butylamine-agarose column chromatography and DEAE-Sephacel column chromatography
-
Ni-NTA agarose column chromatography, Poros Q column chromatography, and Sephadex G-75 gel filtration
-
partial purification
-
recombinant enzyme from Escherichia coli by heat treatment at 85°C for 15 min, dialysis, cation exchange chromatography, and hydrophobic interaction chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Gold by nickel affinity chromatography, tag cleavage through thrombin, dialysis, and gel filtration
recombinant isolated DHO and DDHO-ATC complex from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant mutant His-tagged enzyme by nickel affinity chromatography and dialysis
recombinant N-terminal His-SUMO-tagged enzyme SaPyrC from Escherichia coli strain BL21(DE3), by nickel affinity chromatography, dialysis, and His-SUMO tag cleavage through HRV 3C protease, another step of nickel affinity chromatography to remove the tag, followed by ultrafiltration, and gel filtration, to about 95% purity, method optimization, the His-SUMO tag affects enzyme activity slightly
recombinant protein purified to homogeneity