3.5.2.3: dihydroorotase
This is an abbreviated version!
For detailed information about dihydroorotase, go to the full flat file.
Word Map on EC 3.5.2.3
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3.5.2.3
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pyrimidine
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transcarbamoylase
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carbamoyl-phosphate
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phosphoribosyltransferase
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atcase
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orotidine
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carbamoyltransferase
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glutamine-dependent
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l-dihydroorotate
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2.1.3.2
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cpsase
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pyre
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dihydropyrimidinase
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hydantoinase
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dihydro-ouabain
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n-phosphonacetyl-l-aspartate
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n-carbamyl-l-aspartate
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allantoinase
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imidase
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synthesis
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succinate-grown
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amidohydrolases
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6.3.5.5
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drug development
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medicine
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5-fluoroorotate
- 3.5.2.3
- pyrimidine
-
transcarbamoylase
- carbamoyl-phosphate
- phosphoribosyltransferase
- atcase
- orotidine
-
carbamoyltransferase
-
glutamine-dependent
- l-dihydroorotate
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2.1.3.2
- cpsase
-
pyre
- dihydropyrimidinase
- hydantoinase
- dihydro-ouabain
- n-phosphonacetyl-l-aspartate
-
n-carbamyl-l-aspartate
- allantoinase
- imidase
- synthesis
-
succinate-grown
-
amidohydrolases
-
6.3.5.5
- drug development
- medicine
- 5-fluoroorotate
Reaction
Synonyms
amidohydrolase family protein, BcDHOase, CAD, carbamoylaspartic dehydrase, Class I DHOase, DHO, DHOase, dihydroorotase, dihydroorotase domain, dihydroorotate dehydrolase, hDHOase, huDHOase, human DHOase domain, LdDHOase, More, pyrC, type I DHOase, type II DHO, VcDHO, YpDHO
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Purification
Purification on EC 3.5.2.3 - dihydroorotase
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dihydroorotase and aspartate transcarbamoylase copurify, partially purified by anion exchange chromatography, gel filtration, hydrophobic interaction chromatography
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HiTrap Q column chromatography, Hiload Superdex gel filtration, and phenyl-Superose HR column chromatography
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N-linked butylamine-agarose column chromatography and DEAE-Sephacel column chromatography
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Ni-NTA agarose column chromatography, Poros Q column chromatography, and Sephadex G-75 gel filtration
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recombinant enzyme from Escherichia coli by heat treatment at 85°C for 15 min, dialysis, cation exchange chromatography, and hydrophobic interaction chromatography
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Gold by nickel affinity chromatography, tag cleavage through thrombin, dialysis, and gel filtration
recombinant isolated DHO and DDHO-ATC complex from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant mutant His-tagged enzyme by nickel affinity chromatography and dialysis
recombinant N-terminal His-SUMO-tagged enzyme SaPyrC from Escherichia coli strain BL21(DE3), by nickel affinity chromatography, dialysis, and His-SUMO tag cleavage through HRV 3C protease, another step of nickel affinity chromatography to remove the tag, followed by ultrafiltration, and gel filtration, to about 95% purity, method optimization, the His-SUMO tag affects enzyme activity slightly
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration