Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
dihydroorotase, electro-elution, SDS-PAGE and Coomassie staining | Deinococcus radiophilus |
498000 | - |
gel filtration, DHOase in complex with aspartate transcarbamoylase | Deinococcus radiophilus |
500000 | - |
aspartate transcarbamoylase-dihydroorotase complex, gel filtration | Deinococcus radiophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiophilus | - |
- |
- |
Deinococcus radiophilus NCIMB 10648 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
dihydroorotase and aspartate transcarbamoylase copurify, partially purified by anion exchange chromatography, gel filtration, hydrophobic interaction chromatography | Deinococcus radiophilus |
HiTrap Q column chromatography, Hiload Superdex gel filtration, and phenyl-Superose HR column chromatography | Deinococcus radiophilus |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, pH 8.0 | Deinococcus radiophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + H2O | aspartate transcarbamoylase-dihydroorotase complex | Deinococcus radiophilus | N-carbamoyl-L-aspartate | - |
r | |
(S)-dihydroorotate + H2O | aspartate transcarbamoylase-dihydroorotase complex | Deinococcus radiophilus NCIMB 10648 | N-carbamoyl-L-aspartate | - |
r | |
L-dihydroorotate + H2O | - |
Deinococcus radiophilus | N-carbamoyl-L-aspartate | - |
? | |
L-dihydroorotate + H2O | - |
Deinococcus radiophilus NCIMB 10648 | N-carbamoyl-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Deinococcus radiophilus |
hexamer | 6 * 49000, SDS-PAGE | Deinococcus radiophilus |
Synonyms | Comment | Organism |
---|---|---|
DHOase | - |
Deinococcus radiophilus |
type I DHOase | - |
Deinococcus radiophilus |