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3.5.2.3: dihydroorotase

This is an abbreviated version!
For detailed information about dihydroorotase, go to the full flat file.

Word Map on EC 3.5.2.3

Reaction

(S)-dihydroorotate
+
H2O
=
N-carbamoyl-L-aspartate

Synonyms

amidohydrolase family protein, BcDHOase, CAD, carbamoylaspartic dehydrase, Class I DHOase, DHO, DHOase, dihydroorotase, dihydroorotase domain, dihydroorotate dehydrolase, hDHOase, huDHOase, human DHOase domain, LdDHOase, More, pyrC, type I DHOase, type II DHO, VcDHO, YpDHO

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.3 dihydroorotase

Storage Stability

Storage Stability on EC 3.5.2.3 - dihydroorotase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1 month, 40% loss in activity
-
-20°C, activity is stable idefinitely
Betapolyomavirus macacae
-
-20°C, Na HEPES, pH 7.3, 10% (v/v) glycerol
-
-20°C, Tris-HCl buffer, pH 8.0
-
-70°C, HEPES buffer, pH 7.4, 0.5% glycerol
-80°C, Bis-Tris propane buffer, pH 7.0, 20% glycerol
4°C, 2 months, partially purified preparation
-
4°C, Na HEPES, pH 7.5
-
4°C, pH 7.6, phosphate buffer 0.01 - 0.1 M, 3 days
-
4°C, under nitrogen, 100 mM Tris phosphate buffer, pH 7, metal free 10 mM N-carbamoyl-DL-aspartate, at least 2 months
-