Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain X7014a | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
x-ray structure | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D250A | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
D250E | reduced catalytic activity compared to wild type | Escherichia coli |
D250H | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
D250N | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
D250S | reduced catalytic activity compared to wild type | Escherichia coli |
H254N | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
N44A | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
R20K | reduced catalytic activity compared to wild type | Escherichia coli |
R20M | catalytically inactive | Escherichia coli |
R20Q | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carbamoyl aspartate | mutants D250A, D250H, D250N, R20Q, R20M, N44A, H254N inactive | Escherichia coli | |
0.009 | - |
thiodihydroorotate | Cd/Cd-dihydroorotase, pH 8.0 | Escherichia coli | |
0.03 | - |
thiodihydroorotate | Zn/Zn-dihydroorotase, pH 8.0 | Escherichia coli | |
0.08 | - |
dihydroorotate | Zn/Zn-dihydroorotase, pH 8.0 | Escherichia coli | |
0.23 | - |
dihydroorotate | Cd/Cd-dihydroorotase, pH 8.0 | Escherichia coli | |
0.51 | - |
carbamoyl aspartate | mutant D250S | Escherichia coli | |
0.7 | - |
dihydroorotate | Co/Co-dihydroorotase, pH 8.0 | Escherichia coli | |
0.9 | - |
carbamoyl aspartate | mutant R20K | Escherichia coli | |
1.7 | - |
carbamoyl aspartate | wild type, Zn/Zn-dihydroorotase, pH 5.8 | Escherichia coli | |
1.9 | - |
carbamoyl aspartate | mutant D250E | Escherichia coli | |
4 | - |
carbamoyl aspartate | Cd/Cd-dihydroorotase, pH 5.8 | Escherichia coli | |
15 | - |
carbamoyl aspartate | Co/Co-dihydroorotase, pH 5.8 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Escherichia coli | |
Co2+ | - |
Escherichia coli | |
Zn2+ | activity of Zn2+ > Co2+ > Cd2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05020 | - |
- |
Purification (Comment) | Organism |
---|---|
chromatography, gel filtration, resource-Q anion exchange column | Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, Bis-Tris propane buffer, pH 7.0, 20% glycerol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
N-carbamoyl-L-aspartate | - |
Escherichia coli | (S)-dihydroorotate + H2O | - |
r | |
thio-dihydroorotate + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHO | - |
Escherichia coli |
pyrC | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.9 | synthesis reaction | Escherichia coli |
7.8 | 8.5 | hydrolysis reaction | Escherichia coli |