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Literature summary for 3.5.2.3 extracted from

  • Anderson, M.A.; Cleland, W.W.; Huang, D.T.; Chan, C.; Shojaei, M.; Christopherson, R.I.
    13C and 15N isotope effects for conversion of L-dihydroorotate to N-carbamyl-L-aspartate using dihydroorotase from hamster and Bacillus caldolyticus (2006), Biochemistry, 45, 7132-7139.
    View publication on PubMed
Search for more literature for 3.5.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain SO1263 Mesocricetus auratus
expressed in Escherichia coli strain SO1263 [Bacillus] caldolyticus
overexpression in Escherichia coli strain BL21(DE3) [Bacillus] caldolyticus
overexpression in Escherichia coli strain SØ1263/pyrC- Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
-
-
[Bacillus] caldolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mesocricetus auratus
-
 [Bacillus] caldolyticus
more than 95% purified by PorosHQ column [Bacillus] caldolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + H2O
-
 Mesocricetus auratus N-carbamoyl-L-aspartate
-
 r
(S)-dihydroorotate + H2O
-
 [Bacillus] caldolyticus N-carbamoyl-L-aspartate
-
 r
N-carbamoyl-L-aspartate
-
 Mesocricetus auratus L-dihydroorotate + H2O
-
 r
N-carbamoyl-L-aspartate
-
 [Bacillus] caldolyticus L-dihydroorotate + H2O
-
 r

Synonyms

Synonyms Comment Organism
BcDHOase
-
 [Bacillus] caldolyticus
DHOase
-
 Mesocricetus auratus
DHOase
-
 [Bacillus] caldolyticus
hDHOase
-
 Mesocricetus auratus
pyrC
-
 [Bacillus] caldolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45
-
 Mesocricetus auratus
25 45
-
 [Bacillus] caldolyticus
25 45 differences in kinetic isotope effects due to temperature change are nonexistent for hDHOase even though both systems are run at suboptimal temperatures Mesocricetus auratus
37 60
-
 Mesocricetus auratus
37 60
-
 [Bacillus] caldolyticus
37 60 kinetic isotope effects due to temperature change show a very moderate difference in the secondary kinetic isotope effect for BcDHOase even though both systems are run at suboptimal temperatures [Bacillus] caldolyticus