Cloned (Comment) | Organism |
---|---|
gene pyrC, recombinant enzyme expression in Escherichia coli strain Rosetta-gami 2(DE3), subcloning in Escherichia coli strain DH5alpha | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetic analysis, recombinant enzyme | Methanocaldococcus jannaschii | |
0.14 | - |
(S)-dihydroorotate | pH 8.3, 25°C, recombinant enzyme | Methanocaldococcus jannaschii | |
0.15 | - |
(S)-dihydroorotate | pH 8.3, 45°C, recombinant enzyme | Methanocaldococcus jannaschii | |
0.52 | - |
(S)-dihydroorotate | pH 8.3, 80°C, recombinant enzyme | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc-dependent metalloenzyme, average of 2.05 mol zinc per mol protein classifying the Methanocaldococcus jannaschii DHOase as a two-zinc center protein | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + H2O | Methanocaldococcus jannaschii | - |
N-carbamoyl-L-aspartate | - |
r | |
(S)-dihydroorotate + H2O | Methanocaldococcus jannaschii ATCC 624773 | - |
N-carbamoyl-L-aspartate | - |
r | |
N-carbamoyl-L-aspartate | Methanocaldococcus jannaschii | - |
(S)-dihydroorotate + H2O | - |
r | |
N-carbamoyl-L-aspartate | Methanocaldococcus jannaschii ATCC 624773 | - |
(S)-dihydroorotate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
i.e. Methanococcus jannaschii | - |
Methanocaldococcus jannaschii ATCC 624773 | - |
i.e. Methanococcus jannaschii | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by heat treatment at 85°C for 15 min, dialysis, cation exchange chromatography, and hydrophobic interaction chromatography | Methanocaldococcus jannaschii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal growth of the organism of 85°C | Methanocaldococcus jannaschii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + H2O | - |
Methanocaldococcus jannaschii | N-carbamoyl-L-aspartate | - |
r | |
(S)-dihydroorotate + H2O | - |
Methanocaldococcus jannaschii ATCC 624773 | N-carbamoyl-L-aspartate | - |
r | |
N-carbamoyl-L-aspartate | - |
Methanocaldococcus jannaschii | (S)-dihydroorotate + H2O | - |
r | |
N-carbamoyl-L-aspartate | - |
Methanocaldococcus jannaschii ATCC 624773 | (S)-dihydroorotate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 47503-48104, sequence calculation, mass spectrometry, and SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
DHOase | - |
Methanocaldococcus jannaschii |
pyrC | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | assay at | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 90 | purified recombinant enzyme, 160 min, stable | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.3 | assay at | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
metabolism | catalyzes the conversion of N-carbamoyl-L-aspartate to dihydroorotate in the third step of the de novo biosynthesis of pyrimidines | Methanocaldococcus jannaschii |
additional information | structure homology modelling using the structure of DHOase from Methanocaldococcus jannaschii strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440, UniProt ID Q58885. Sequence and structure comparisons | Methanocaldococcus jannaschii |