3.5.1.49: formamidase

This is an abbreviated version!
For detailed information about formamidase, go to the full flat file.

Word Map on EC 3.5.1.49

Reaction

Formamide
+
H2O
=
formate
+
NH3

Synonyms

Fmd, Formamide amidohydrolase, formamidase, acetamidase/formamidase, Amds/fmds, formamidase AmiF, AmiF formamidase, formamide aminohydrolase, AmiF, TE19, HpyAmiF, BceAmiF

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.49 formamidase

Molecular Weight

Molecular Weight on EC 3.5.1.49 - formamidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
123000
-
gel filtration
133000 - 137000
-
gel filtration, non-denaturing PAGE
135000 - 160000
-
gel filtration
160000
-
gel filtration
180000
SDS-PAGE, the 180000 Da purified protein yields a heat-denatured species of 45000 Da
191000
purified formamidase, native PAGE
32000
4 * 32000, calculated from amino acid sequence
37300
-
4 * 3400, SDS-PAGE, 4 * 37300, deduced from gene sequence
38000
-
4 * 38000, His-tagged enzyme, SDS-PAGE
38632
-
4 * 38632, His-tagged enzyme, calculated from amino acid sequence
43300
4 * 43300, native state, native PAGE
44481
-
3 * 44481, electrospray mass spectrometry
45000
49770
calculated from amino acid sequence
51000
-
alpha2, 2 * 51000, SDS-PAGE
52000
recombinant His-tagged enzyme