3.5.1.49: formamidase

This is an abbreviated version!
For detailed information about formamidase, go to the full flat file.

Reaction

Synonyms

acetamidase/formamidase, Amds/fmds, AmiF, AmiF formamidase, BceAmiF, Fmd, formamidase, formamidase AmiF, Formamide amidohydrolase, formamide aminohydrolase, HpyAmiF, TE19

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.49 formamidase

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Results	in table
1	Activating Compound
2395	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
4	Crystallization (Commentary)
22	General Information
6	Inhibitors
1	Ki Value [mM]
6	KM Value [mM]
5	Localization
3	Metals/Ions
18	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
36	Organism
5	Pathway
30	PDB ID
5	pH Optimum
2	pH Range
1	pI Value
18	Protein Variants
9	Purification (Commentary)
1	Reaction
4	Reaction Type
32	Reference
14	Source Tissue
26	Specific Activity [micromol/min/mg]
44	Substrates and Products (Substrate)
18	Subunits
40	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
2	Temperature Range [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 3.5.1.49 - formamidase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in the soluble form by Escherichia coli XL1 Blue MRF cells (5mg fusion protein per liter culture), purified on a Glutathione Sepharose 4B Resin column, cleaved with thrombin

Paracoccidioides brasiliensis

Q8J0S3

675813

glutatione Sepharose column chromatography, DEAE Sepharose column chromatography, and phenyl Sepharose column chromatography

Paracoccidioides brasiliensis

C1GWV9

712044

MagneHis protein purification system

Lupinus albus

B9VXW6

713326

Ni-NTA His-binding affinity chromatography, gel filtration on a Sephacryl S-200 column

Caldanaerobacter subterraneus subsp. tengcongensis

Q8R8S5

671137

recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration

Bacillus cereus

P59701

752598

recombinant protein

Helicobacter pylori

-

209149

Talon metal-affinity resin column chromatography and Superdex 200 gel filtration

Bacillus cereus

-

718640

to homogeneity

Methylophilus methylotrophus

-

209144, 209145