3.5.1.49: formamidase
This is an abbreviated version!
For detailed information about formamidase, go to the full flat file.
Word Map on EC 3.5.1.49
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3.5.1.49
-
kynurenine
-
urease
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2,3-dioxygenase
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n-formyl-l-kynurenine
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2-aminofluorene
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methylotrophus
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methylophilus
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n-formylation
- 3.5.1.49
- kynurenine
- urease
-
2,3-dioxygenase
- n-formyl-l-kynurenine
- 2-aminofluorene
- methylotrophus
- methylophilus
-
n-formylation
Reaction
Synonyms
acetamidase/formamidase, Amds/fmds, AmiF, AmiF formamidase, BceAmiF, Fmd, formamidase, formamidase AmiF, Formamide amidohydrolase, formamide aminohydrolase, HpyAmiF, TE19
ECTree
3.5.1.49 formamidaseAdvanced search results
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results (Cloned
Cloned on EC 3.5.1.49 - formamidase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
Escherichia coli strain Rosetta (DE3), 20-residue His-tag at N-terminus
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
gene amiF, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
into the SalI/NotI sites of the Vector pGEX-4T-3, fused to glutathione S-transferase (GST)
the native soluble protein is expressed in Escherichia coli Rosetta (DE3) cells, the SeMet-substituted protein is expressed in the methionine-auxotrophic Escherichia coli strain B834 (DE3)
