Literature summary for 3.5.1.49 extracted from

Qian, M.; Huang, Q.; Wu, G.; Lai, L.; Tang, Y.; Pei, J.; Kusunoki, M.
Crystal structure analysis of a recombinant predicted acetamidase/formamidase from the thermophile Thermoanaerobacter tengcongensis (2012), Protein J., 31, 166-174.

Search for more literature for 3.5.1.49

Cloned(Commentary)

Cloned (Comment)	Organism
the native soluble protein is expressed in Escherichia coli Rosetta (DE3) cells, the SeMet-substituted protein is expressed in the methionine-auxotrophic Escherichia coli strain B834 (DE3)	Caldanaerobacter subterraneus subsp. tengcongensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.2 M calcium acetate, 0.1 M sodium cacodylate and 18% (w/v) PEG 8000 pH 6.2 for the native protein or 18% (w/v) PEG 8000, 0.2 M calcium acetate and 0.1 M sodium acetate pH 5.8 for the SeMet-substituted protein	Caldanaerobacter subterraneus subsp. tengcongensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	contains 5 Ca2+ ions	Caldanaerobacter subterraneus subsp. tengcongensis
Zn2+	contains 4 Zn2+ ions	Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	4 * 32000, calculated from amino acid sequence	Caldanaerobacter subterraneus subsp. tengcongensis

Organism

Organism	UniProt	Comment	Textmining
Caldanaerobacter subterraneus subsp. tengcongensis	Q8R8S5	-	-

Subunits

Subunits	Comment	Organism
homotetramer	4 * 32000, calculated from amino acid sequence	Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

Synonyms	Comment	Organism
acetamidase/formamidase	-	Caldanaerobacter subterraneus subsp. tengcongensis
Amds/fmds	-	Caldanaerobacter subterraneus subsp. tengcongensis
TE19	-	Caldanaerobacter subterraneus subsp. tengcongensis

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Release 2023.1 (January 2023)