Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
E140D | the mutation does not significantly alter enzyme folding and shows no detectable activity | Bacillus cereus |
W136H | this mutation causes the absence of overexpression of the enzyme | Bacillus cereus |
Y191F | the mutation does not significantly alter enzyme folding and shows 11.8% activity compared to the wild type enzyme | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at concentrations above 15 mM | Bacillus cereus | |
iodoacetamide | complete inhibition at concentrations above 15 mM | Bacillus cereus | |
additional information | the presence of EDTA and dithiothreitol has no significant effect on enzyme activity | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
108 | - |
Formamide | in 100 mM sodium citrate buffer (pH 6.0), at 50°C | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the presence of K+, Na+, Ca2+, Mg2+, and Pb2+ has no significant effect on enzyme activity | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, His-tagged enzyme, SDS-PAGE | Bacillus cereus |
38632 | - |
4 * 38632, His-tagged enzyme, calculated from amino acid sequence | Bacillus cereus |
135000 | 160000 | gel filtration | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetamide + H2O | Bacillus cereus | - |
acetate + NH3 | - |
? | |
acetamide + H2O | Bacillus cereus CECT 5050T | - |
acetate + NH3 | - |
? | |
formamide + H2O | Bacillus cereus | best substrate | formate + NH3 | - |
? | |
formamide + H2O | Bacillus cereus CECT 5050T | best substrate | formate + NH3 | - |
? | |
additional information | Bacillus cereus | propionamide, butyramide, isobutyramide, alaninamide, leucinamide, glycinamide, and urea are not recognized as substrates | ? | - |
? | |
additional information | Bacillus cereus CECT 5050T | propionamide, butyramide, isobutyramide, alaninamide, leucinamide, glycinamide, and urea are not recognized as substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus CECT 5050T | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal-affinity resin column chromatography and Superdex 200 gel filtration | Bacillus cereus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetamide + H2O | - |
Bacillus cereus | acetate + NH3 | - |
? | |
acetamide + H2O | - |
Bacillus cereus CECT 5050T | acetate + NH3 | - |
? | |
formamide + H2O | best substrate | Bacillus cereus | formate + NH3 | - |
? | |
formamide + H2O | best substrate | Bacillus cereus CECT 5050T | formate + NH3 | - |
? | |
additional information | propionamide, butyramide, isobutyramide, alaninamide, leucinamide, glycinamide, and urea are not recognized as substrates | Bacillus cereus | ? | - |
? | |
additional information | propionamide, butyramide, isobutyramide, alaninamide, leucinamide, glycinamide, and urea are not recognized as substrates | Bacillus cereus CECT 5050T | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 38000, His-tagged enzyme, SDS-PAGE | Bacillus cereus |
homotetramer | 4 * 38632, His-tagged enzyme, calculated from amino acid sequence | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
AmiF | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | about 50% activity at 35 and 70°C | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | after incubation at the optimal temperature for activity of the enzyme (50°C) for 40 h, the enzyme maintains around 95% of its initial activity. Activity is gradually lost when the enzyme is incubated at temperatures over 65°C for 30 min. The melting temperature is at 73.5°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
maximum activity in citrate and cacodylate buffers at pH 6.0. Using sodium phosphate as the buffering agent as opposed to cacodylate/citrate, a decrease in enzyme activity is observed at pH 6.0 | Bacillus cereus |