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Literature summary for 3.5.1.49 extracted from
- Identification of genes induced in proteoid roots of white lupin under nitrogen and phosphorus deprivation, with functional characterization of a formamidase (2010), Plant Soil, 334, 137-150.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) cells | Lupinus albus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 56 | - |
Formamide | native formamidase, pH and temperature not specified in the publication | Lupinus albus |  |
| 71 | - |
Formamide | recombinant formamidase, pH and temperature not specified in the publication | Lupinus albus | |
| 120 | - |
Propionamide | recombinant formamidase, pH and temperature not specified in the publication | Lupinus albus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49770 | - |
calculated from amino acid sequence | Lupinus albus |
| 52000 | - |
recombinant His-tagged enzyme | Lupinus albus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus albus | B9VXW6 | white lupin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MagneHis protein purification system | Lupinus albus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Lupinus albus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formamide + H2O | - |
Lupinus albus | formate + NH3 | - |
? | |
| additional information | formamidase shows no activity with nicotinamide | Lupinus albus | ? | - |
? | |
| propionamide + H2O | - |
Lupinus albus | propionate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fmd | - |
Lupinus albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant formamidase | Lupinus albus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | recombinant formamidase | Lupinus albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Lupinus albus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Lupinus albus |
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Release 2023.1 (January 2023)
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