3.5.1.41: chitin deacetylase
This is an abbreviated version!
For detailed information about chitin deacetylase, go to the full flat file.
Reaction
chitin +
H2O=
chitosan +
acetate
Synonyms
AN9380.2, AnCDA, ANIA_09380, ArCE4, Blcda, BmCDA6, BmCDA7, BmCDA8, CDA, CDA-like protein, CDA1, Cda1p, CDA2, cda2aa, cda2b, Cda2p, CDA3, cda4, cda5a, cda5b, cdaYJ, CE4 deacetylase, CHD, chitin amidohydrolase,, chitin deacetylase, chitin deacetylase 1, chitin deacetylase 2, chitin deacetylase 2A, chitin deacetylase 2B, chitin deacetylase 3, chitin deacetylase 4, chitin deacetylase 5A, chitin deacetylase 5B, chitin deacetylase 6, chitin deacetylase 7, chitin deacetylase 8, chitin deacetylase 9, chitin deacetylase RC, chitin deacetylase-like protein, chitin deacetylase2, ChlD, ClCDA, D2 chitin deacetylase, Fpd1, FV-PDA, Hacda2, I3/2 chitin deacetylase, LmCDA1, LmCDA2, LmCDA2a, LmCDA2b, LmCDA4, LmCDA5a, LmCDA5b, midgut chitin deacetylase, More, MrCDA, N-acetylglucosamine deacetylase, NodB, NodB deacetylase, NodB homology domain-containing protein, PaCDA, PcCDA, pc_2566, PesCDA, PgtCDA, PODANS_1_4780, RC chitin deacetylase, ScCDA2, TcCDA1, TcCDA2A, TcCDA2B, TcCDA3, TcCDA4, TcCDA5A, TcCDA5B, TcCDA6, TcCDA7, TcCDA8, TcCDA9, VcCDA, VpCDA
ECTree
Specific Activity
Specific Activity on EC 3.5.1.41 - chitin deacetylase
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0.042
-
intracellular enzyme activity of strain IFM 40505 after 5 days of growth
0.044
-
intracellular enzyme activity of strain URM 2136 after 5 days of growth
0.047
-
intracellular enzyme activity after 2 days of growth
0.06
-
intracellular enzyme activity after 2 days of growth
0.061
substrate peritrophic matrix chitin, isozyme BmCDA8, pH 7.4, 30°C
0.075
-
intracellular enzyme activity of strain IFM 46114 after 5 days of growth
0.078
substrate colloidal chitin, isozyme BmCDA6, pH 7.4, 30°C
0.2
recombinant His6-tagged enzyme in Pichia pastoris cell culture supernatant
0.207
substrate colloidal chitin, isozyme BmCDA8, pH 7.4, 30°C
0.284
substrate peritrophic matrix chitin, isozyme BmCDA7, pH 7.4, 30°C
0.393
substrate colloidal chitin, isozyme BmCDA7, pH 7.4, 30°C
0.63
-
crude extract, at pH 9.0 and 50°C
135.6
purified recombinant His6-tagged enzyme, substrate penta-N-acetyl-chitopentaose
138.5
purified recombinant His6-tagged enzyme, substrate tetra-N-acetyl-chitotetraose
175
-
after 58fold purification, using water-soluble chitin with degree of deacetylation of 50% as substrate, at pH 6.0 and 50°C
184.4
purified recombinant His6-tagged enzyme, substrate hexa-N-acetyl-chitohexaose
2.58
-
substrate: hexa-N-acetylchitohexaose
3
-
culture filtrate, using water-soluble chitin with degree of deacetylation of 50% as substrate, at pH 6.0 and 50°C
55.91
-
purified native enzyme, substrate colloidal chitin, pH 6.5, 50°C
0.018
-
pH 6.0, 28°C
0.018
Cunninghamella ramosa
-
intracellular enzyme activity of strain URM 1918 after 5 days of growth
55.38
-
after 88.25fold purification, at pH 9.0 and 50°C
55.38
-
purified enzyme, pH 9.0, 50°C
additional information
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additional information
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additional information
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the specific activity using glycol chitin as substrate is 4.17 units/mg
additional information
enzymatic activity as micromol acetate/min/micromol of protein
additional information
enzymatic activity as micromol acetate/min/micromol of protein
additional information
enzymatic activity as micromol acetate/min/micromol of protein
additional information
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enzymatic activity as micromol acetate/min/micromol of protein
additional information
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-
additional information
substrate specificity
additional information
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substrate specificity
additional information
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no activity detectable in cells grown for 2 days
additional information
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no activity detectable in cells grown for 2 days
additional information
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no activity detectable in cells grown for 2 days
additional information
Cunninghamella ramosa
-
no activity detectable in cells grown for 2 days
additional information
purified reombinant isozyme CDA1, 0.268 U/microliter
additional information
purified reombinant isozyme CDA1, 0.268 U/microliter
additional information
purified reombinant isozyme CDA1, 0.268 U/microliter
additional information
purified reombinant isozyme CDA2a, 0.354 U/microliter
additional information
purified reombinant isozyme CDA2a, 0.354 U/microliter
additional information
purified reombinant isozyme CDA2a, 0.354 U/microliter
additional information
purified reombinant isozyme CDA2b, 0.228 U/microliter
additional information
purified reombinant isozyme CDA2b, 0.228 U/microliter
additional information
purified reombinant isozyme CDA2b, 0.228 U/microliter
additional information
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no activity detectable in cells grown for 5 days
additional information
activity of recombinant PgtCDA produced in different bacterial expression strains with chitin hexamer at pH 8.0 and 37°C, overview
additional information
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activity of recombinant PgtCDA produced in different bacterial expression strains with chitin hexamer at pH 8.0 and 37°C, overview
additional information
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no activity detectable in cells grown for 5 days
additional information
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3.5.1.41 chitin deacetylase
results (
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