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Literature summary for 3.5.1.41 extracted from
- Purification and characterization of chitin deacetylase from Penicillium oxalicum SAEM-51 (2012), Carbohydr. Polym., 87, 1091-1097.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | sodium acetate at 80-100 mM results in 30-80% loss in activity; while 50% of the activity is retained at 90 mM concentration | Penicillium oxalicum |  |
| Bi2+ | inhibitory at 1 mM | Penicillium oxalicum | |
| EDTA | inhibitory at 1 mM | Penicillium oxalicum | |
| Mo2+ | inhibitory at 1 mM | Penicillium oxalicum | |
| additional information | no inhibition by acetate; not inhibited by up to 60 mM acetate | Penicillium oxalicum | |
| Pb2+ | inhibitory at 1 mM | Penicillium oxalicum | |
| Zn2+ | inhibitory at 1 mM | Penicillium oxalicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Penicillium oxalicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium oxalicum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Penicillium oxalicum | |
| Cd2+ | activates | Penicillium oxalicum | |
| Co2+ | activates | Penicillium oxalicum | |
| Cu2+ | activates | Penicillium oxalicum | |
| Fe2+ | activates | Penicillium oxalicum | |
| Mg2+ | activates | Penicillium oxalicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Penicillium oxalicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Penicillium oxalicum | - |
chitosan + acetate | - |
? | |
| chitin + H2O | Penicillium oxalicum SAEM-51 | - |
chitosan + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium oxalicum | - |
- |
- |
| Penicillium oxalicum | - |
the strain is developed by mutagenesis from wild-type strain ITCC 6965, isolated from residual materials from sea food processing industry, Saubhagya Seafoods, Porbandar Gujarat, India | - |
| Penicillium oxalicum SAEM-51 | - |
- |
- |
| Penicillium oxalicum SAEM-51 | - |
the strain is developed by mutagenesis from wild-type strain ITCC 6965, isolated from residual materials from sea food processing industry, Saubhagya Seafoods, Porbandar Gujarat, India | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CM-Sepharose column chromatography and DEAE-Sepharose column chromatography | Penicillium oxalicum |
| native extracellular enzyme 88.25fold to homogeneity by ultrafiltration, cation and anion exchange chromatography | Penicillium oxalicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.63 | - |
crude extract, at pH 9.0 and 50°C | Penicillium oxalicum |
| 55.38 | - |
after 88.25fold purification, at pH 9.0 and 50°C | Penicillium oxalicum |
| 55.38 | - |
purified enzyme, pH 9.0, 50°C | Penicillium oxalicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Penicillium oxalicum | chitosan + acetate | - |
? | |
| chitin + H2O | 100% activity | Penicillium oxalicum | chitosan + acetate | - |
? | |
| chitin + H2O | the enzyme deacetylates glycol chitin and chitin oligomers having degree of polymerization of more than four | Penicillium oxalicum | chitosan + acetate | - |
? | |
| chitin + H2O | - |
Penicillium oxalicum SAEM-51 | chitosan + acetate | - |
? | |
| chitin + H2O | 100% activity | Penicillium oxalicum SAEM-51 | chitosan + acetate | - |
? | |
| chitin + H2O | the enzyme deacetylates glycol chitin and chitin oligomers having degree of polymerization of more than four | Penicillium oxalicum SAEM-51 | chitosan + acetate | - |
? | |
| di-N-acetylchitobiose + H2O | marginal activity, 16.3% activity compared to glycol chitin | Penicillium oxalicum | ? | - |
? | |
| additional information | the enzyme is not able to deacetylate N-acetyl glucosamine | Penicillium oxalicum | ? | - |
? | |
| additional information | no activity with N-acetyl glucosamine, marginal activity towards (GlcNAc)2 and (GlcNAc)3 | Penicillium oxalicum | ? | - |
? | |
| additional information | the enzyme is not able to deacetylate N-acetyl glucosamine | Penicillium oxalicum SAEM-51 | ? | - |
? | |
| additional information | no activity with N-acetyl glucosamine, marginal activity towards (GlcNAc)2 and (GlcNAc)3 | Penicillium oxalicum SAEM-51 | ? | - |
? | |
| penta-N-acetylchitopentaose + H2O | highly active, 121.8% activity compared to glycol chitin | Penicillium oxalicum | ? | - |
? | |
| tri-N-acetylchitotriose + H2O | marginal activity, 25.94% activity compared to glycol chitin | Penicillium oxalicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Penicillium oxalicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDA | - |
Penicillium oxalicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Penicillium oxalicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | activity range, profile overview | Penicillium oxalicum |
| 40 | 70 | about 50% activity at 40 and 70°C | Penicillium oxalicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermodynamics of enzyme denaturation, overview | Penicillium oxalicum |
| 50 | - |
the enzyme has a half-life of 693.10 min at 50°C | Penicillium oxalicum |
| 50 | - |
purified enzyme, pH 9.0, half-life is 693.10 min | Penicillium oxalicum |
| 60 | - |
purified enzyme, pH 9.0, 80% activity remaning after 180 min | Penicillium oxalicum |
| 70 | - |
purified enzyme, pH 9.0, 50% activity remaning after 180 min | Penicillium oxalicum |
| 80 | - |
purified enzyme, pH 9.0, inactivation after 120 min | Penicillium oxalicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Penicillium oxalicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | purified enzyme, 24 h, 25-55% activity remaining | Penicillium oxalicum |
| 5 | 9 | the enzyme retains 100% of its activity at pH 9.0 after 24 h of incubation. Further, it retains about 25-55% of its activity when incubated with buffers of comparatively lower pH of 5.0-7.0 | Penicillium oxalicum |
| 9 | - |
purified enzyme, 24 h, completely stable | Penicillium oxalicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penicillium oxalicum | isoelectric focusing | - |
5.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme structure shows presence of 56.26% alpha-helical and 15.63% beta-helical structures, structure comparison, overview | Penicillium oxalicum |
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