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3.5.1.41: chitin deacetylase

This is an abbreviated version!
For detailed information about chitin deacetylase, go to the full flat file.

Word Map on EC 3.5.1.41

Reaction

chitin
+
H2O
=
chitosan
 +
acetate

Synonyms

AN9380.2, AnCDA, ANIA_09380, ArCE4, Blcda, BmCDA6, BmCDA7, BmCDA8, CDA, CDA-like protein, CDA1, Cda1p, CDA2, cda2aa, cda2b, Cda2p, CDA3, cda4, cda5a, cda5b, cdaYJ, CE4 deacetylase, CHD, chitin amidohydrolase,, chitin deacetylase, chitin deacetylase 1, chitin deacetylase 2, chitin deacetylase 2A, chitin deacetylase 2B, chitin deacetylase 3, chitin deacetylase 4, chitin deacetylase 5A, chitin deacetylase 5B, chitin deacetylase 6, chitin deacetylase 7, chitin deacetylase 8, chitin deacetylase 9, chitin deacetylase RC, chitin deacetylase-like protein, chitin deacetylase2, ChlD, ClCDA, D2 chitin deacetylase, Fpd1, FV-PDA, Hacda2, I3/2 chitin deacetylase, LmCDA1, LmCDA2, LmCDA2a, LmCDA2b, LmCDA4, LmCDA5a, LmCDA5b, midgut chitin deacetylase, More, MrCDA, N-acetylglucosamine deacetylase, NodB, NodB deacetylase, NodB homology domain-containing protein, PaCDA, PcCDA, pc_2566, PesCDA, PgtCDA, PODANS_1_4780, RC chitin deacetylase, ScCDA2, TcCDA1, TcCDA2A, TcCDA2B, TcCDA3, TcCDA4, TcCDA5A, TcCDA5B, TcCDA6, TcCDA7, TcCDA8, TcCDA9, VcCDA, VpCDA

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.41 chitin deacetylase

Purification

Purification on EC 3.5.1.41 - chitin deacetylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
110fold to homogeneity
-
140fold, partial
-
516fold to homogeneity
-
944fold to homogeneity
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Sephacryl S-300 gel filtration, and Resource Q column chromatography
-
CM-Sepharose column chromatography and DEAE-Sepharose column chromatography
-
functional recombinant solubilized and refolded C-terminally Strep-tagged CDA catalytic domain expressed in Escherichia coli strain BL21(DE3) by affinity chromatography, dialysis, and ultrafiltration
-
His-Bind column chromatography
-
native enzyme 3.94fold by ammonium sulfate precipitation, ion exchange chromatography, and gel filtration
-
native extracellular enzyme 88.25fold to homogeneity by ultrafiltration, cation and anion exchange chromatography
-
native extracellular enzyme partially by ammonium sulfate fractionation and dialysis
-
partially from fungal culture medium, from solid state and submerged culture
-
recombinant and original CDA enzymes, to homogeneity
-
recombinant enzyme
-
recombinant extracellular His-tagged enzyme from Pichia pastoris strain X-33 culture medium by ultrafiltration, nickel affinity chromatography
recombinant His-tagged CDA2 from Saccharomyces cerevisiae strain BY4741 by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli pLysS by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity and anion exchange chromatography
recombinant His-tagged isozyme BmCDA6 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography
recombinant His-tagged isozyme BmCDA7 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography
recombinant His-tagged isozyme BmCDA8 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography
recombinant His-tagged isozyme Cda1 by nickel affinity chromatography
-
recombinant His-tagged isozyme Cda2 by nickel affinity chromatography
-
recombinant His6-tagged enzyme 364fold from Pichia pastoris strain GS115 culture supernatant by the single step process of nickel affinity chromatography
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity
A0A1U8QU02
recombinant Strep-tagged enzyme from Hansenula polymorpha strain RB11 by StrepTactin affinity chromatography
separation of several chitin degrading enzymes from culture medium supernatant by ion exchange chromatography
-
to homogeneity
-