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3.5.1.41: chitin deacetylase

This is an abbreviated version!
For detailed information about chitin deacetylase, go to the full flat file.

Word Map on EC 3.5.1.41

Reaction

chitin
+
H2O
=
chitosan
 +
acetate

Synonyms

AN9380.2, AnCDA, ANIA_09380, ArCE4, Blcda, BmCDA6, BmCDA7, BmCDA8, CDA, CDA-like protein, CDA1, Cda1p, CDA2, cda2aa, cda2b, Cda2p, CDA3, cda4, cda5a, cda5b, cdaYJ, CE4 deacetylase, CHD, chitin amidohydrolase,, chitin deacetylase, chitin deacetylase 1, chitin deacetylase 2, chitin deacetylase 2A, chitin deacetylase 2B, chitin deacetylase 3, chitin deacetylase 4, chitin deacetylase 5A, chitin deacetylase 5B, chitin deacetylase 6, chitin deacetylase 7, chitin deacetylase 8, chitin deacetylase 9, chitin deacetylase RC, chitin deacetylase-like protein, chitin deacetylase2, ChlD, ClCDA, D2 chitin deacetylase, Fpd1, FV-PDA, Hacda2, I3/2 chitin deacetylase, LmCDA1, LmCDA2, LmCDA2a, LmCDA2b, LmCDA4, LmCDA5a, LmCDA5b, midgut chitin deacetylase, More, MrCDA, N-acetylglucosamine deacetylase, NodB, NodB deacetylase, NodB homology domain-containing protein, PaCDA, PcCDA, pc_2566, PesCDA, PgtCDA, PODANS_1_4780, RC chitin deacetylase, ScCDA2, TcCDA1, TcCDA2A, TcCDA2B, TcCDA3, TcCDA4, TcCDA5A, TcCDA5B, TcCDA6, TcCDA7, TcCDA8, TcCDA9, VcCDA, VpCDA

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.41 chitin deacetylase

Cloned

Cloned on EC 3.5.1.41 - chitin deacetylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CDA2, phylogenetic analysis, functional expression as His-tagged protein in Saccharomyces cerevisiae strain BY4741
CDA6 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns
CDA7 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns
CDA8 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
expressed in Pichia pastoris
-
expression of His6-tagged enzyme in Pichia pastoris strain GS115, secretion of the recombinant enzyme to the culture supernatant
expression of His8-tagged isozymes in Escherichia coli strain BL21
-
expression of the C-terminally His6-tagged enzyme in Pichia pastoris and in Escherichia coli, the His-tag causes intramolecular interactions with the active site
gene ANIA_09380, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21
A0A1U8QU02
gene Blcda, metagenomics library construction from the soil exposed to chitin and chitosan yielding chitin modifying enzymes, DNA and amino acid sequence determination and analysis, genotyping, recombinant expression in Escherichia coli strain Rosetta 2(DE3)
-
gene Blcda, recombinant expression of His-tagged enzyme in Escherichia coli pLysS, method optimization, optimized medium contains 2% glycerol, 10 g/l yeast extract and 1 g/l NaCl
-
gene BmCDA7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Pichia pastoris strain GS115, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene cda1+ or SPAC19G12.03, interaction analysis using the two-hybrid system in Saccharomyces cerevisiae strain L40
gene cda1, recombinant expression of His-tagged enzyme in Spodoptera frugiperda Sf9 cells via transfection with the baculovirus method
gene cda1, sequence comparison with ClCDA from Colletotrichum lindemuthianum, recombinant expression in Escherichia coli and secretion into the periplasmic space of the bacterial host, recombinant expression of MBP-tagged, StrepII-tagged, or His-tagged enzyme in Escherichia coli strains BL21(DE3), ER2508, or Rosetta 2(DE3), optimal expression in Escherichia coli strain Rosetta2(DE3)/pLysSRARE as MBP-tagged enzyme
gene CDA2, sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33, the enzyme is secreted
gene cda2a, recombinant expression of His-tagged enzyme in Spodoptera frugiperda Sf9 cells via transfection with the baculovirus method
gene CDA2a, two splicing variants a and b exist, sequence comparisons, gene structure analysis, and phylogenetic analysis and tree, recombinant expression in Escherichia coli
gene cda2b, recombinant expression of His-tagged enzyme in Spodoptera frugiperda Sf9 cells via transfection with the baculovirus method
gene CDA2b, two splicing variants a and b exist, sequence comparisons, gene structure analysis, and phylogenetic analysis and tree, recombinant expression in Escherichia coli
gene cda4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis
gene cda5a, two splicing variants of LmCDA5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis
gene cda5b, two splicing variants of LmCDA5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis
gene CfCDA2a, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, quantitative real-time RT-PCR expression analysis
gene CfCDA2b, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, quantitative real-time RT-PCR expression analysis
gene Hacda2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis
gene nodB, recombinant overexpression of His-tagged enzyme NodB in Escherichia coli strain BL21(DE3), recombinant expression of Trx-NodB. The recombinant enzyme expressed in a soluble and catalytically active form is then efficiently used as biocatalyst
gene pc_2566, sequence comparisons and phylogenetic tree, functional recombinant expression of C-terminally StrepII-tagged CDA catalytic domain in Escherichia coli strain BL21(DE3) in inclusion bodies
-
identification and characterization of a chitin deacetylase from a metagenomic library of deep-sea sediments of the arctic ocean, genotyping, phylogenetic analysis of CdaYJ, cdaYJ gene is subcloned into the pET-28a vector and recombinantly overexpressed in Escherichia coli strain BL21(DE3)
-
into the yeast expression vector pPICZalphaA
overexpression of full-length and truncated enzyme versions as GFP-tagged proteins in recombinant embryos, overview
-
phylogenetic analysis
phylogenetic tree, recombinant expression of Strep-tagged enzyme in Hansenula polymorpha strain RB11
recombinant expression in Pichia pastoris
-
recombinant expression of His-tagged isozyme Cda1
-
recombinant expression of His-tagged isozyme Cda2
-
recombinant expression of the enzyme in Hansenula polymorpha as a full length protein composed of the CE4 domain flanked by two CBM18 domains
-
the chitin deacetylase gene is subcloned into a pET28a expression vector and expressed in Escherichia coli BL21 cells
-
three chitin deacetylase cDNAs (RC, D2, and I3/2) are isolated from the cDNA library and sequenced. These cDNAs exhibit features characteristic of chitin deacetylase sequences: the presence of a polysaccharide deacetylase domain, a metal-binding triad, the conserved catalytic residues, and high homology with various chitin deacetylase genes. The cDNAs are cloned in a Pichia pastoris expression system and produced as polyhistidine-tagged proteins. Only recombinant enzyme chitin deacetylase RC is active under the tested conditions
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