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Literature summary for 3.5.1.41 extracted from
- Purification and characterization of chitin deacetylase from Colletotrichum lindemuthianum (1995), J. Biol. Chem., 270, 26286-26291.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Colletotrichum lindemuthianum |  |
| Mn2+ | - |
Colletotrichum lindemuthianum | |
| Zn2+ | - |
Colletotrichum lindemuthianum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Colletotrichum lindemuthianum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | substrate: glycol chitosan | Colletotrichum lindemuthianum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
enzyme after deglycosylation, gel filtration, SDS-PAGE | Colletotrichum lindemuthianum |
| 150000 | - |
amino acid and carbohydrate analysis in combination with data from SDS-PAGE and gel filtration | Colletotrichum lindemuthianum |
| 250000 | - |
gel filtration, SDS-PAGE | Colletotrichum lindemuthianum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Colletotrichum lindemuthianum | - |
chitosan + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colletotrichum lindemuthianum | - |
DSM 63144 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | galactose, mannose, xylose, fucose, N-acetyl-D-glucosamine, N-acetylgalactosamine, N-acetylneuraminic acid | Colletotrichum lindemuthianum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 110fold to homogeneity | Colletotrichum lindemuthianum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Colletotrichum lindemuthianum | - |
| mycelium | - |
Colletotrichum lindemuthianum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.219 | - |
- |
Colletotrichum lindemuthianum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C | Colletotrichum lindemuthianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylchitin + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
| chitin + H2O | - |
Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
| chitin + H2O | amorphous chitin | Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
| chitin + H2O | chitin 50 | Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
| glycol chitin + H2O | - |
Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
| glycol chitosan + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
| N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
| N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
| N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
| N,N',N''-triacetylchitotriose + H2O | high substrate concentration needed | Colletotrichum lindemuthianum | ? | - |
? | |
| N,N'-diacetylchitobiose + H2O | high substrate concentration needed | Colletotrichum lindemuthianum | deacetylated chitooligosaccharides + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 150000, amino acid and carbohydrate analysis in combination with data from SDS-PAGE and gel filtration | Colletotrichum lindemuthianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Colletotrichum lindemuthianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Colletotrichum lindemuthianum |
| 50 | - |
nearly stable for 45 h | Colletotrichum lindemuthianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Colletotrichum lindemuthianum |
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