3.4.24.72: fibrolase
This is an abbreviated version!
For detailed information about fibrolase, go to the full flat file.
Word Map on EC 3.4.24.72
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3.4.24.72
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plasmin
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thrombolytic
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copperhead
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fibrinogenolytic
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medicine
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nonhemorrhagic
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neuwiedi
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alteplase
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pharmacology
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drug development
- 3.4.24.72
- plasmin
-
thrombolytic
- copperhead
-
fibrinogenolytic
- medicine
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nonhemorrhagic
- neuwiedi
- alteplase
- pharmacology
- drug development
Reaction
Hydrolysis of -Ala14-/-Leu- in insulin B chain and -Lys413-/-Leu- in Aalpha-chain of fibrinogen =
Synonyms
3-203-fibrolase[3-serine], AfeE, Agkistrodon contortrix contortrix metalloproteinase, ALF, Alfimeprase, brevilysin L6, brevithrombolase, broad bean-fibrinolytic enzyme, CFR15-protease, fibrinolytic enzyme, fibrinolytic protease, Fibrinolytic proteinase, FIIa, neuwiedase, Non-hemorrhagic fibrinolytic metalloproteinase, plasmin, Proteinase, Agkistrodon contortrix contortrix venom metallo-, snake venom fibrinolytic enzyme, starase, UFEIII, VlF, wulfase
ECTree
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Cloned
Cloned on EC 3.4.24.72 - fibrolase
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chimeric Arg-Gly-Asp-fibrolase is expressed in Escherichia coli BL21(DE3) cells
co-expression of the active signal-less His-tagged enzyme with chaperone DsbC in Escherichia coli cytoplasm, over 90% of the recombinant enzyme is soluble due to DsbC, while the chaperones FkpA and Skp do not have an effect on fibrolase solubility
expression of the wild-type fibrolase and a truncated enzyme form, alfimeprase
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gene alf, high level expression of His-tagged alfimeprase in Pichia pastoris strains of GS115 and KM71 by methanol induction, secretion of the recombinant enzyme to the cell culture medium