3.4.24.72: fibrolase
This is an abbreviated version!
For detailed information about fibrolase, go to the full flat file.
Word Map on EC 3.4.24.72
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3.4.24.72
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plasmin
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thrombolytic
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copperhead
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fibrinogenolytic
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medicine
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nonhemorrhagic
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neuwiedi
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alteplase
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pharmacology
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drug development
- 3.4.24.72
- plasmin
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thrombolytic
- copperhead
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fibrinogenolytic
- medicine
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nonhemorrhagic
- neuwiedi
- alteplase
- pharmacology
- drug development
Reaction
Hydrolysis of -Ala14-/-Leu- in insulin B chain and -Lys413-/-Leu- in Aalpha-chain of fibrinogen =
Synonyms
3-203-fibrolase[3-serine], AfeE, Agkistrodon contortrix contortrix metalloproteinase, ALF, Alfimeprase, brevilysin L6, brevithrombolase, broad bean-fibrinolytic enzyme, CFR15-protease, fibrinolytic enzyme, fibrinolytic protease, Fibrinolytic proteinase, FIIa, neuwiedase, Non-hemorrhagic fibrinolytic metalloproteinase, plasmin, Proteinase, Agkistrodon contortrix contortrix venom metallo-, snake venom fibrinolytic enzyme, starase, UFEIII, VlF, wulfase
ECTree
3.4.24.72 fibrolaseAdvanced search results
results (
results (Purification
Purification on EC 3.4.24.72 - fibrolase
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PURIFICATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and DEAE-Sephadex gel filtration
ammonium sulfate precipitation and Poros-HQ ion-exchange column chromatography and Sephadex G100 gel filtration
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ammonium sulfate precipitation, DEAE-cellulose ion-exchange column chromatography and Sephadex G-75 gel filtration
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ammonium sulfate precipitation, dialysis and DEAE-cellulose column chromatography
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ammonium sulfate precipitation, Hi Prep phenyl column chromatography, and Superdex 75 gel filtration
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ammonium sulfate precipitation, HPD-100 macroporous resin column chromatography, 201 × 7 ion-exchange resin column chromatography, and Superdex 200 gel filtration
ammonium sulfate precipitation, octyl Sepharose column chromatography, SP Sepharose column chromatography, Sephadex G-25 gel filtration, and Superdex 75 gel filtration
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aqueous two-phase system with 30% (w/v) polyethylene glycol 6000 and 13.2% (w/v) sodium sulfate
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DEAE Sepharose column chromatography and Q Sepharose column chromatography
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DEAE-Sepharose CL6B column chromatography, Sepharose CL4B column chromatography, and Mono Q HR column chromatography
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high performance liquid chromatography, hydroxyapatite high performance liquid chromatography and cation exchange high performance liquid chromatography
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HiTrap Q-Sepharose column chromatography, gel filtration
hydrophobic interaction high performance liquid chromatography, hydroxyapatite high performance liquid chromatography and cation exchange high performance liquid chromatography
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hydrophobic interaction HPLC, hydroxyapatite HPLC and cation exchange HPLC
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native enzyme by hydrophobic interaction and hydroxyapatite chromatography
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Q-Sepharose column chromatography, Sephacryl S-100 gel filtration, and Sephadex G-50 gel filtration
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recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity and hydrophobic interaction chromatography to 95.6% purity
secreted recombinant His-tagged alfimeprase from Pichia pastoris strains of GS115 and KM71 cell culture medium by immunoaffinity chromatography to 96% purity
