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4-amidinophenylmethylsulfonyl fluoride
-
5% residual activity at 2 mM
4-bromophenacyl bromide
-
78.5% residual activity at 2 mM
acetaldehyde
-
about 3% activity at 2 mM
acetone
-
complete inhibition at 10% (v/v)
aprotin
84.1% residual activity at 1 mM
-
Ba2+
-
79.86% residual activity at 10 mM
bovine serum albumin
-
complete inhibition at 1% (w/v)
-
Cs2+
-
89.14% residual activity at 10 mM
CTAB
-
about 75% residual activity at 2 mM
cysteine
-
47.4% residual activity at 20 mM
disodium-EDTA
-
21.1% residual activity at 4 mM
DMSO
-
98.14% residual activity at 10 mM
ethanol
-
about 90% residual activity at 2 mM
Glutaraldehyde
-
about 5% residual activity at 2 mM
iodoacetamide
-
49.9% residual activity at 4 mM
iodoacetate
-
17.08% residual activity at 2 mM
L-lysine
-
38.5% residual activity at 5 mM
methanol
-
about 95% residual activity at 2 mM
N-4-tosyl-phenylalanyl chloromethyl ketone
-
16.5% residual activity at 0.1 mM
n-butanol
-
about 75% residual activity at 2 mM
Nalpha-tosyl-L-lysine chloromethylketone
-
51.5% residual activity at 0.1 mM
oxidized glutathione
-
complete inhibition at 5 mM
Pb2+
-
85.5% inhibition at 10 mM
pepstatin
-
71.5% residual activity at 2.5 mM
peptone
-
61.5% residual activity at 1% (w/v)
-
phenylmethane sulfonyl fluoride
-
-
phenylmethyl sulfonyl fluoride
-
about 90% residual activity at 2 mM
phenylmethylsulfonyl fluoride
protein SBTI
-
26% residual activity at 4 mM
-
reduced glutathione
-
complete inhibition at 5 mM
Soybean trypsin inhibitor
-
tetraethylene pentamine
-
rapid and complete inhibition
tosyl-L-lysine chloromethyl ketone
21.3% residual activity at 0.1 mM
tosyl-L-phenylalanine chloromethyl ketone
37.2% residual activity at 0.1 mM
tosyl-phenylalanine chloromethyl ketone
Triton X-100
-
87% residual activity at 0.01% (v/v)
Tween 20
-
80% residual activity at 0.01% (v/v)
Urea
-
complete inhibition at 8 M
1,10-phenanthroline
-
complete and rapid inhibition
1,10-phenanthroline
-
rapid and complete inhibition
1,10-phenanthroline
-
17.48% residual activity at 2 mM
2-mercaptoethanol
-
36.2% residual activity at 5 mM
2-mercaptoethanol
-
95.08% residual activity at 10 mM
2-mercaptoethanol
-
about 10% residual activity at 2 mM
2-mercaptoethanol
-
about 65% residual activity at 2 mM
Al3+
-
3.5% residual activity at 10 mM
Al3+
-
about 65% residual activity at 2 mM
Al3+
-
29% residual activity at 5 mM
Al3+
-
about 80% residual activity at 2 mM
alpha2-Macroglobulin
-
-
-
alpha2-Macroglobulin
-
the 720 kDa tetrameric inhibitor causes rapid and irreversible inhibition by sterical hindrance, the PEG-bound enzyme is less sensitive
-
alpha2-Macroglobulin
-
rapid, irreversible inhibition by physical entrapment
-
alpha2-Macroglobulin
-
-
-
Aprotinin
-
strong inhibition
Aprotinin
-
82.3% residual activity at 5 mM
Ca2+
-
87.04% residual activity at 10 mM
Ca2+
-
about 30% residual activity at 2 mM
Ca2+
-
88.8% residual activity at 1 mM
Co2+
-
48.3% residual activity at 5 mM
Co2+
-
21.6% inhibition at 10 mM
Co2+
-
61.59% residual activity at 10 mM
Co2+
60.4% residual activity at 1 mM
Co2+
-
60.8% residual activity at 1 mM
Co2+
-
about 80% residual activity at 2 mM
Cu2+
-
50% residual activity at 5 mM
Cu2+
-
4.55% residual activity at 10 mM
Cu2+
-
54.09% residual activity at 10 mM
Cu2+
-
61.2% residual activity at 5 mM
Cu2+
-
5.0% residual activity at 5 mM
Cu2+
50.1% residual activity at 1 mM
Cu2+
-
34.2% residual activity at 1 mM
Cu2+
-
32% residual activity at 5 mM
Cu2+
-
about 55% residual activity at 2 mM
dithiothreitol
-
84.92% residual activity at 10 mM
dithiothreitol
-
75% residual activity at 10 mM
dithiothreitol
-
92.8% residual activity at 4 mM
EDTA
-
complete and rapid inhibition
EDTA
-
EDTA sensitivity of the isoforms of the natural and of the recombinant enzyme
EDTA
-
rapid and complete inhibition
EDTA
-
10.4% residual activity at 5 mM
EDTA
-
2.15% residual activity at 10 mM
EDTA
-
67.85% residual activity at 5 mM
EDTA
-
13.5% residual activity at 5 mM
EDTA
-
16.51% residual activity at 2 mM
EDTA
-
6.25% residual activity at 5 mM
EDTA
77.5% residual activity at 5 mM
EDTA
-
39.9% residual activity at 10 mM
EDTA
-
45% residual activity at 1 mM
EDTA
-
complete inhibition at 2 mM
EGTA
-
strong inhibition
EGTA
74.7% residual activity at 5 mM
EGTA
-
36% residual activity at 1 mM
EGTA
-
complete inhibition at 2 mM
Fe2+
-
6.82% residual activity at 5 mM
Fe2+
-
52.95% residual activity at 10 mM
Fe2+
77.3% residual activity at 1 mM
Fe2+
-
95.3% residual activity at 1 mM
Fe2+
-
70% residual activity at 5 mM
Fe2+
-
complete inhibition at 2 mM
Fe3+
-
complete inhibition at 5 mM
Fe3+
-
about 40% residual activity at 2 mM
Fe3+
-
95.4% residual activity at 1 mM
Fe3+
-
36% residual activity at 5 mM
Hg2+
-
63% inhibition at 10 mM
Hg2+
-
17% residual activity at 5 mM
iodoacetic acid
-
80.54% residual activity at 10 mM
iodoacetic acid
-
96.42% residual activity at 10 mM
K+
-
98.6% residual activity at 5 mM
K+
-
86.69% residual activity at 10 mM
K+
-
30.9% inhibition at 10 mM
K+
-
93.86% residual activity at 2.5 mM
K+
-
about 80% residual activity at 2 mM
leupeptin
98.6% residual activity at 1 mM
leupeptin
-
about 75% residual activity at 2 mM
Mg2+
-
96.8% residual activity at 5 mM
Mg2+
-
92.64% residual activity at 10 mM
Mg2+
-
about 70% residual activity at 2 mM
Mg2+
-
94.4% residual activity at 1 mM
Mg2+
-
about 75% residual activity at 2 mM
Mn2+
-
strong inhibition
Mn2+
94.2% residual activity at 1 mM
Mn2+
-
about 70% residual activity at 2 mM
Na+
-
84.41% residual activity at 10 mM
Na+
-
91.59% residual activity at 2.5 mM
Na+
97.7% residual activity at 1 mM
Na+
-
about 85% residual activity at 2 mM
pepstatin A
-
48.2% residual activity at 5 mM
pepstatin A
-
84.31% residual activity at 10 mM
pepstatin A
-
91.3% residual activity at 5 mM
pepstatin A
95.2% residual activity at 1 mM
pepstatin A
-
14.6% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
8.48% residual activity at 5 mM
phenylmethylsulfonyl fluoride
-
8.62% residual activity at 10 mM
phenylmethylsulfonyl fluoride
-
42.85% residual activity at 5 mM
phenylmethylsulfonyl fluoride
-
52.9% residual activity at 2 mM
phenylmethylsulfonyl fluoride
-
complete inhibition at 5 mM
phenylmethylsulfonyl fluoride
-
4% residual activity at 2 mM
phenylmethylsulfonyl fluoride
18.5% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
25.4% residual activity at 50 mM
phenylmethylsulfonyl fluoride
-
28% residual activity at 4 mM
phenylmethylsulfonyl fluoride
-
about 70% residual activity at 2 mM
SDS
-
29% residual activity at 0.01% (w/v)
SDS
-
complete inhibition at 0.1% (w/v)
Soybean trypsin inhibitor
-
complete inhibition at 2 mM
-
Soybean trypsin inhibitor
28.2% residual activity at 1 mM
-
Tetraethylenepentamine
-
complete and rapid inhibition
Tetraethylenepentamine
-
-
tosyl-phenylalanine chloromethyl ketone
-
76.2% residual activity at 5 mM
tosyl-phenylalanine chloromethyl ketone
-
about 80% residual activity at 2 mM
Zn2+
-
52.6% residual activity at 5 mM
Zn2+
-
30% residual activity at 10 mM
Zn2+
-
about 80% residual activity at 2 mM
Zn2+
-
11% residual activity at 5 mM
Zn2+
67.6% residual activity at 1 mM
Zn2+
-
81.4% residual activity at 1 mM
Zn2+
-
42% residual activity at 5 mM
Zn2+
-
complete inhibition at 2 mM
additional information
-
not inhibited by any serine protease inhibitor
-
additional information
-
not: DFP, soybean trypsin inhibitor, trasylol, PCMB
-
additional information
-
polyethylene glycol addition to the enzyme modifies the enzyme size and reduces its activity
-
additional information
-
not inhibited by EDTA, iodoacetic acid, and 2-mercaptoethanol
-
additional information
-
not inhibited by EDTA and EGTA
-
additional information
-
not inhibited by iodoacetic acid
-
3.4.24.72 fibrolase
results (
results (
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