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3.4.21.1: chymotrypsin

This is an abbreviated version!
For detailed information about chymotrypsin, go to the full flat file.

Word Map on EC 3.4.21.1

Reaction

Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/- =

Synonyms

4CHA, Alcalase, alpha chymar, alpha chymotrypsin, alpha-Chy, alpha-chymar ophth, alpha-chymotrypsin, alpha-chymotrypsin A, alpha-CT, avazyme, bovine alpha-chymotrypsin, caldecrin, cationic chymotrypsin, cellulomonadin, CHT, Chtp, Chtr1, Chtr2, Chtr3, Chtr4, ChTRP, CHY1, CHY20, chymar, chymotest, chymotrypsin, chymotrypsin A, chymotrypsin B, chymotrypsin C, chymotrypsin C1, chymotrypsin I, chymotrypsin II, chymotrypsin isoform Kh1, chymotrypsin isoform Kh2, chymotrypsin isoform Kh3, chymotrypsin-B, CTRA, Ctrb, EC 3.4.4.5, EC 3.4.4.6, enzeon, LBCP, lysosomal Bid cleavage protease, PEG-alpha-chymotrypsin, PEG-modified alpha-chymotrypsin, quimar, quimotrase, serine protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.1 chymotrypsin

KI Value

KI Value on EC 3.4.21.1 - chymotrypsin

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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.08
(Cbz-alanyl)aminomethyl boronic acid
-
37°C, pH 8.0
1.96
(Cbz-phenylalanyl)aminomethyl boronic acid
-
37°C, pH 8.0
0.00001
2,2'-[[(1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphoryl]bis(oxybenzene-4,1-diyl)]diacetic acid
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0027
2,2'-[[(1-[[1-(tert-butoxycarbonyl)-L-prolyl]amino]-3-methylbutyl)phosphoryl]bis(oxybenzene-3,1-diyl)]diacetic acid
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0301
2-(2,6-difluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0277
2-(2,6-dimethoxyphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0091
2-(2-bromophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.00047
2-(2-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0125
2-(2-methoxyphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0098
2-(2-methylphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0224
2-(2-nitrophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0091
2-(2-thienyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0226
2-(3-chlorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0178
2-(3-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0161
2-(3-methylphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.01619
2-(3-methylphenyl)-7-nitro-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.341
2-(3-nitrophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.094
2-(4-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0126
2-(4-methoxyphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0164
2-(4-methylphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.03
2-oxo-2-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.75
2-oxo-2-(piperidin-1-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.0087
2-phenyl-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
31.01
4-fluorophenylboronic acid
-
37°C, pH 8.0
0.3363
5'-demethoxyepiexcelsin
-
-
0.303
7-chloro-2-(2-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.00057
Ac-Phe-Val-Thr-p-amino-L-phenylalanine-CHO
-
-
0.0000032
Ac-Phe-Val-Thr-p-nitro-L-phenylalanine-CHO
-
-
0.000011
Ac-Phe-Val-Thr-Phe-CHO
-
-
0.001
Ac-Phe-Val-Thr-pyridyl-L-alanine-CHO
-
-
0.0000017
Ac-Phe-Val-Thr-Tyr-CHO
-
-
0.00001
Acacia plumosa trypsin inhibitor A
-
in phosphate buffer saline, pH 7.4, at 37°C for 30 min
-
0.0000102
Acacia plumosa trypsin inhibitor B
-
in phosphate buffer saline, pH 7.4, at 37°C for 30 min
-
0.0000109
Acacia plumosa trypsin inhibitor C
-
in phosphate buffer saline, pH 7.4, at 37°C for 30 min
-
0.00000000035
anti-trypsin-chymotrypsin A
-
-
-
0.0000000004
anti-trypsin-chymotrypsin B
-
-
-
0.0000075 - 0.000018
Aprotinin
0.00000099
AVPGSWPW
-
-
0.344
benzyloxycarbonyl-Ala-Ala-Phe-glyoxal
-
at 25°C
0.000025 - 0.024
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
0.065
benzyloxycarbonyl-Phe-glyoxal
-
-
0.00085
benzyloxycarbonyl-Pro-Phe-glyoxal
-
37°, pH 7.4
0.00002
bis(2,3-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.02
bis(2-methylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.015
bis(3,4,5-trimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0062
bis(3,4-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00014
bis(3-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0015
bis(4-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.000045
bis(4-methoxyphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0013
bis(4-tert-butylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0033
bis[4-(propan-2-yl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00065
bis[4-(sulfanylmethyl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.11
brein 3-O-myristate
-
-
0.11
brein 3-O-palmitate
-
-
0.0026
chymostatin
pH 7.6, 30°C
0.014
complex of vanadate and benzohydroxamic acid
in 20 mM sodium cacodylate (pH 7.4) and 75% saturated ammonium sulfate
0.42
cycloart-24-ene-3beta-ol
-
-
0.06
dammara-20,24-dien-3beta-ol
-
-
0.0047
diphenyl (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.02229
epiexcelsin
-
-
0.03
faradiol 3-O-myristate
-
-
0.058
faradiol 3-O-palmitate
-
-
0.0047
GAW
-
-
0.000000027
IVNGEEAVPGSWPW
-
-
0.00013 - 0.00017
Lima bean trypsin inhibitor
-
0.057
lup-20(29)-ene-3beta,16beta-diol
-
-
0.026
maniladiol 3-O-myristate
-
-
0.12
maniladiol 3-O-palmitate
-
-
0.0052
micropeptin 478-A
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.072
micropeptin 478-B
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.1
micropeptin HU1021
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.1
micropeptin HU1041
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.1
micropeptin HU1069
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0196
micropeptin HU895A
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0054
micropeptin HU895B
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0028
micropeptin HU909
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.024
micropeptin HU975
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0182
micropeptin HU989
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.00001
mixed monolayer protected gold cluster
-
-
-
0.012
N-(tert-butoxycarbonyl)-L-alanyl-N-[4-[2-(3-oxoprop-1-yn-1-yl)phenyl]but-3-yn-1-yl]-L-phenylalaninamide
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.00005
N-(tert-butoxycarbonyl)-L-valyl-N-(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00036
N-(tert-butoxycarbonyl)-L-valyl-N-[1-(diphenoxyphosphoryl)-2-phenylethyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00033
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00179
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00075
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-tert-butylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.003432
N-acetyl-L-Phe-Ala-Thr-Pal aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.000323
N-acetyl-L-Phe-Ala-Thr-Phe aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.000159
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.000281
N-acetyl-L-Phe-Ala-Thr-Tyr aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.0000112
N-tosyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
9.7
phenylboronic acid
-
37°C, pH 8.0
0.00067 - 0.0019
Soybean trypsin inhibitor
-
0.000074
Suc-Val-Pro-PheP(OPh)2
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.063
taraxast-20(39)-ene-3beta,16beta-diol
-
-
0.068
taraxast-20-ene-3beta,16beta-diol
-
-
0.0005
tert-butyl (2S)-2-([1-[bis(2-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0046
tert-butyl (2S)-2-([1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0108
tert-butyl (2S)-2-([1-[bis(3,4-dimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.02
tert-butyl (2S)-2-([1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.02
tert-butyl (2S)-2-([1-[bis(4-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.073
tert-butyl (2S)-2-[(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)carbamoyl]pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0104
tert-butyl (2S)-2-[[1-(diphenoxyphosphoryl)-2-phenylethyl]carbamoyl]pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.003
tert-butyl [(2S)-1-oxo-3-phenyl-1-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)propan-2-yl]carbamate
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.072
tirucalla-7,24-dien-3beta-ol
-
-
0.000034
TPGVY
-
-
0.11
urs-12-ene-3beta,16beta-diol
-
-
0.00000188
winged bean chymotrypsin-trypsin inhibitor
-
Dixon plot analysis, same for recombinant inhibitor and inhibitor obtained from winged bean seeds
-
0.0000072 - 0.0000094
WSCI