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3.4.21.1: chymotrypsin

This is an abbreviated version!
For detailed information about chymotrypsin, go to the full flat file.

Word Map on EC 3.4.21.1

Reaction

Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/- =

Synonyms

4CHA, Alcalase, alpha chymar, alpha chymotrypsin, alpha-Chy, alpha-chymar ophth, alpha-chymotrypsin, alpha-chymotrypsin A, alpha-CT, avazyme, bovine alpha-chymotrypsin, caldecrin, cationic chymotrypsin, cellulomonadin, CHT, Chtp, Chtr1, Chtr2, Chtr3, Chtr4, ChTRP, CHY1, CHY20, chymar, chymotest, chymotrypsin, chymotrypsin A, chymotrypsin B, chymotrypsin C, chymotrypsin C1, chymotrypsin I, chymotrypsin II, chymotrypsin isoform Kh1, chymotrypsin isoform Kh2, chymotrypsin isoform Kh3, chymotrypsin-B, CTRA, Ctrb, EC 3.4.4.5, EC 3.4.4.6, enzeon, LBCP, lysosomal Bid cleavage protease, PEG-alpha-chymotrypsin, PEG-modified alpha-chymotrypsin, quimar, quimotrase, serine protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.1 chymotrypsin

KM Value

KM Value on EC 3.4.21.1 - chymotrypsin

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 1.4
4-nitrophenyl acetate
0.032 - 0.098
4-nitrophenyl benzoate
7.27
acetyl-L-Leu methyl ester
-
-
0.17
acetyl-L-Trp ethyl ester
-
-
1
acetyl-Leu methyl ester
-
-
1.04 - 1.39
acetyl-Phe ethyl ester
1.12
acetyl-Trp ethyl ester
-
-
1.27 - 2
Acetyl-Tyr ethyl ester
0.59 - 6.79
Ala-Ala-Phe-7-amido-4-methylcoumarin
0.44
benzoyl-4-guanidinophenyl ester
-
25°C, pH 7.6
4.94
benzoyl-D-Ala-4-guanidinophenyl ester
-
25°C, pH 7.6
1.49
benzoyl-D-Leu-4-guanidinophenyl ester
-
25°C, pH 7.6
2.58
benzoyl-D-Phe-4-guanidinophenyl ester
-
25°C, pH 7.6
2.81
benzoyl-Gly-4-guanidinophenyl ester
-
25°C, pH 7.6
2.88
benzoyl-L-Ala-4-guanidinophenyl ester
-
25°C, pH 7.6
0.13 - 0.64
benzoyl-Tyr ethyl ester
7.02 - 8.72
N-acetyl-L-leucine methyl ester
0.0891
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid
-
-
0.0101
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid
-
-
0.1131
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid
-
-
0.1203
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid
-
-
0.0635
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid
-
-
0.001
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
0.0191
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid
-
-
0.0598
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
1.12 - 2.58
N-acetyl-L-phenylalanine ethyl ester
0.14 - 0.19
N-acetyl-L-tryptophan ethyl ester
0.57 - 1.17
N-acetyl-L-tyrosine ethyl ester
0.939
N-alpha-benzoyl-DL-arginine-p-nitroanilide
-
-
0.1 - 0.27
N-benzoyl-L-Tyr ethyl ester
0.01 - 0.18
N-benzoyl-L-tyrosine 4-nitroanilide
0.12 - 8.1
N-benzoyl-L-tyrosine ethyl ester
2.23 - 20.6
N-benzoyl-L-tyrosine methyl ester
0.05 - 0.083
N-benzoyl-L-tyrosine-4-nitroanilide
0.067
N-benzoyl-L-tyrosyl-ethyl ester
pH 8.0, 37°C
0.08 - 0.21
N-benzoyl-Tyr p-nitroanilide
0.0009
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
0.0177
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
0.32 - 6.1
N-glutaryl-L-Phe-p-nitroanilide
0.44
N-glutaryl-L-phenylalanine-4-nitroanilide
pH 7.75, 25°C
0.0013 - 0.023
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
0.32 - 3
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
0.0346
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
0.26 - 0.44
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
0.32 - 2.6
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
0.02 - 0.37
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
0.052 - 0.58
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
0.16 - 1.6
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
0.05 - 0.19
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
0.012
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.0074 - 1.1
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.4 - 0.48
N-succinyl-Ala-Phe-Ala
2.5
N-succinyl-Ala-Phe-p-nitroanilide
-
30°C, pH 8.0
0.044
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide
-
at 25°C
0.074
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide
-
in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C
0.14
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 25°C
1.09
N-succinyl-L-Phe-p-nitroanilide
-
at 25°C
0.024 - 0.375
N-succinyl-L-phenylalanine-4-nitroanilide
0.012
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.01 - 0.032
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
2.3 - 5
N-succinyl-Phe-7-amido-4-methylcoumarin
0.09 - 0.1
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
0.62 - 0.64
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester
0.08
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester
-
-
0.4
Nalpha-tosyl-L-Tyr benzyl ester
-
-
0.0297 - 0.044
p-nitrophenyl acetate
0.072
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 9.0, 25°C
0.022 - 0.07
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
0.048 - 0.074
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.012
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
37°C, pH 8.0
0.28
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide
-
pH 8.0, 25°C
0.09
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide
-
at 30°C
0.054
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
pH 8.5, 25°C
0.022 - 0.12
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
0.0005 - 0.8
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
additional information
casein