3.2.1.67: galacturonan 1,4-alpha-galacturonidase

This is an abbreviated version!
For detailed information about galacturonan 1,4-alpha-galacturonidase, go to the full flat file.

Reaction

Synonyms

alkaline exo-polygalacturonase, carrot exopolygalacturonase, CbPelA, corn pollen polygalacturonase, EPG, exo polygalacturonase, exo-D-galacturonanase, exo-D-galacturonase, Exo-PG, exo-PGase, exo-PGI, exo-PGII, exo-PGU B, exo-poly alpha galactouronosidase, exo-polygalacturonanase, exo-polygalacturonase, exolytic PGase, exoPG, exoPG1, exoPG2, exoPGase, exopoly-D-galacturonase, exopolygalacturonanase, exopolygalacturonase, exopolygalacturonase B, exopolygalacturonase X, Galacturan 1,4-alpha-galacturonidase, GH28, macerating enzyme, More, MpPG1, MpPG3, oligogalacturonate hydrolase, peach exopolygalacturonase, PECI, pectinase, PelB, PG III, PGase, PGase I, PGC2, PGC3, PGI, poly 1,4-alpha-D-galacturonide-galacturonohydrolase, Poly(1,4-alpha-D-galacturonide)galacturonohydrolase, poly(galacturonate)hydrolase, poly-(1-4)-alpha-D-galactosiduronate glycanohydrolase, polygalacturonanase, polygalacturonase, polymethylgalacturonase, RPG15, RPG16, SSXPG1, SSXPG2, TM0437, TmGalU, YeGH2

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.67 galacturonan 1,4-alpha-galacturonidase

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Results	in table
17	Activating Compound
284	AA Sequence
29	Application
1	CAS Registry Number
18	Cloned(Commentary)
2	Crystallization (Commentary)
11	Expression
19	General Information
10	General Stability
1	IC50 Value
214	Inhibitors
15	kcat/KM [mM/s]
9	Ki Value [mM]
68	KM Value [mM]
38	Localization
39	Metals/Ions
59	Molecular Weight [Da]
75	Natural Substrates/ Products (Substrates)
171	Organism
3	Pathway
4	PDB ID
79	pH Optimum
26	pH Range
34	pH Stability
17	pI Value
21	Posttranslational Modification
3	Protein Variants
67	Purification (Commentary)
1	Reaction
8	Reaction Type
149	Reference
44	Source Tissue
43	Specific Activity [micromol/min/mg]
5	Storage Stability
370	Substrates and Products (Substrate)
70	Subunits
260	Synonyms
1	Systematic Name
52	Temperature Optimum [°C]
19	Temperature Range [°C]
61	Temperature Stability [°C]
40	Turnover Number [1/s]

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Turnover Number on EC 3.2.1.67 - galacturonan 1,4-alpha-galacturonidase

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.17 - 182

(1,4-alpha-D-galacturonide)2

2 entries

0.44 - 685

(1,4-alpha-D-galacturonide)3

2 entries

0.52 - 829

(1,4-alpha-D-galacturonide)4

2 entries

0.57 - 934

(1,4-alpha-D-galacturonide)5

2 entries

0.59 - 821

(1,4-alpha-D-galacturonide)6

2 entries

0.33 - 860

(1,4-alpha-D-galacturonide)7

2 entries

0.31 - 843

(1,4-alpha-D-galacturonide)8

2 entries

936

(1,4-alpha-D-galacturonide)n

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

770

citric pectin

Aspergillus giganteus

-

pH 6.0, 55°C

709377

-

11.7 - 14.1

digalacturonic acid

2 entries

2.58 - 690

polygalacturonic acid

5 entries

9.55 - 21.9

trigalacturonic acid

2 entries

936

[(1->4)-alpha-D-galacturonide]170

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

-

3.9 - 182

[(1->4)-alpha-D-galacturonide]2

3 entries

10.7 - 685

[(1->4)-alpha-D-galacturonide]3

2 entries

829

[(1->4)-alpha-D-galacturonide]4

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

934

[(1->4)-alpha-D-galacturonide]5

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

821

[(1->4)-alpha-D-galacturonide]6

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

860

[(1->4)-alpha-D-galacturonide]7

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

843

[(1->4)-alpha-D-galacturonide]8

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

393.3 - 4068

[(1->4)-alpha-D-galacturonide]n

4 entries

0.17

(1,4-alpha-D-galacturonide)2

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

182

(1,4-alpha-D-galacturonide)2

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.44

(1,4-alpha-D-galacturonide)3

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

685

(1,4-alpha-D-galacturonide)3

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.52

(1,4-alpha-D-galacturonide)4

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

829

(1,4-alpha-D-galacturonide)4

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.57

(1,4-alpha-D-galacturonide)5

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

934

(1,4-alpha-D-galacturonide)5

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.59

(1,4-alpha-D-galacturonide)6

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

821

(1,4-alpha-D-galacturonide)6

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.33

(1,4-alpha-D-galacturonide)7

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

860

(1,4-alpha-D-galacturonide)7

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.31

(1,4-alpha-D-galacturonide)8

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

843

(1,4-alpha-D-galacturonide)8

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

11.7

digalacturonic acid

Rhodothermus marinus

D0MEP1

at pH 7.0 and 25°C

749629

14.1

digalacturonic acid

Thermotoga thermophilus

-

at pH 6.0 and 40°C

751540

2.58

polygalacturonic acid

Bacillus licheniformis

-

at pH 6.5 and 60°C

751702

6.02

polygalacturonic acid

Rhodothermus marinus

D0MEP1

at pH 7.0 and 25°C

749629

10.6

polygalacturonic acid

Thermotoga thermophilus

-

at pH 6.0 and 40°C

751540

194

polygalacturonic acid

Aspergillus niger

-

at pH 4.0 and 40°C

749439

690

polygalacturonic acid

Aspergillus giganteus

-

pH 6.0, 55°C

709377

9.55

trigalacturonic acid

Thermotoga thermophilus

-

at pH 6.0 and 40°C

751540

21.9

trigalacturonic acid

Rhodothermus marinus

D0MEP1

at pH 7.0 and 25°C

749629

3.9

[(1->4)-alpha-D-galacturonide]2

Rhizopus arrhizus

Q5W9U0

isozyme RPG16, pH 4.0, 30°C

731158

10.3

[(1->4)-alpha-D-galacturonide]2

Rhizopus arrhizus

Q5W9U0

isozyme RPG15, pH 4.0, 30°C

731158

182

[(1->4)-alpha-D-galacturonide]2

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

10.7

[(1->4)-alpha-D-galacturonide]3

Rhizopus arrhizus

Q5W9U0

isozyme RPG15, pH 4.0, 30°C

731158

685

[(1->4)-alpha-D-galacturonide]3

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

393.3

[(1->4)-alpha-D-galacturonide]n

Aspergillus niveus

-

pH 4.0, 50°C

731836

2022

[(1->4)-alpha-D-galacturonide]n

Penicillium oxalicum

-

pH 5.0, 50°C, recombinant catalytic polygalacturonase domain

729080

2230

[(1->4)-alpha-D-galacturonide]n

Rhizopus arrhizus

-

pH 5.0, 50°C

731416

4068

[(1->4)-alpha-D-galacturonide]n

Penicillium oxalicum

-

pH 5.0, 50°C, recombinant wild-type full-length enzyme

729080