3.2.1.67: galacturonan 1,4-alpha-galacturonidase

This is an abbreviated version!
For detailed information about galacturonan 1,4-alpha-galacturonidase, go to the full flat file.

Reaction

Synonyms

alkaline exo-polygalacturonase, carrot exopolygalacturonase, CbPelA, corn pollen polygalacturonase, EPG, exo polygalacturonase, exo-D-galacturonanase, exo-D-galacturonase, Exo-PG, exo-PGase, exo-PGI, exo-PGII, exo-PGU B, exo-poly alpha galactouronosidase, exo-polygalacturonanase, exo-polygalacturonase, exolytic PGase, exoPG, exoPG1, exoPG2, exoPGase, exopoly-D-galacturonase, exopolygalacturonanase, exopolygalacturonase, exopolygalacturonase B, exopolygalacturonase X, Galacturan 1,4-alpha-galacturonidase, GH28, macerating enzyme, More, MpPG1, MpPG3, oligogalacturonate hydrolase, peach exopolygalacturonase, PECI, pectinase, PelB, PG III, PGase, PGase I, PGC2, PGC3, PGI, poly 1,4-alpha-D-galacturonide-galacturonohydrolase, Poly(1,4-alpha-D-galacturonide)galacturonohydrolase, poly(galacturonate)hydrolase, poly-(1-4)-alpha-D-galactosiduronate glycanohydrolase, polygalacturonanase, polygalacturonase, polymethylgalacturonase, RPG15, RPG16, SSXPG1, SSXPG2, TM0437, TmGalU, YeGH2

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.67 galacturonan 1,4-alpha-galacturonidase

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Results	in table
17	Activating Compound
284	AA Sequence
29	Application
1	CAS Registry Number
18	Cloned(Commentary)
2	Crystallization (Commentary)
11	Expression
19	General Information
10	General Stability
1	IC50 Value
214	Inhibitors
15	kcat/KM [mM/s]
9	Ki Value [mM]
68	KM Value [mM]
38	Localization
39	Metals/Ions
59	Molecular Weight [Da]
75	Natural Substrates/ Products (Substrates)
171	Organism
3	Pathway
4	PDB ID
79	pH Optimum
26	pH Range
34	pH Stability
17	pI Value
21	Posttranslational Modification
3	Protein Variants
67	Purification (Commentary)
1	Reaction
8	Reaction Type
149	Reference
44	Source Tissue
43	Specific Activity [micromol/min/mg]
5	Storage Stability
370	Substrates and Products (Substrate)
70	Subunits
260	Synonyms
1	Systematic Name
52	Temperature Optimum [°C]
19	Temperature Range [°C]
61	Temperature Stability [°C]
40	Turnover Number [1/s]

KM Value

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KM Value on EC 3.2.1.67 - galacturonan 1,4-alpha-galacturonidase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.34 - 2.8

(1,4-alpha-D-galacturonide)2

5 entries

0.19 - 0.97

(1,4-alpha-D-galacturonide)3

5 entries

0.1 - 0.29

(1,4-alpha-D-galacturonide)4

2 entries

0.077 - 0.53

(1,4-alpha-D-galacturonide)5

3 entries

0.057 - 0.44

(1,4-alpha-D-galacturonide)6

3 entries

0.029 - 0.32

(1,4-alpha-D-galacturonide)7

3 entries

0.02 - 0.159

(1,4-alpha-D-galacturonide)8

3 entries

0.012 - 6

(1,4-alpha-D-galacturonide)n

7 entries

0.012 - 0.0193

digalacturonic acid

2 entries

0.012 - 0.9

pectic acid

6 entries

0.49

Pectin

Geotrichum lactis

-

-

136791, 136792

0.000673 - 0.00877

polygalacturonic acid

2 entries

0.00241 - 0.0123

trigalacturonic acid

2 entries

0.06

[(1->4)-alpha-D-galacturonide]170

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

-

0.016 - 0.34

[(1->4)-alpha-D-galacturonide]2

3 entries

0.0092 - 0.34

[(1->4)-alpha-D-galacturonide]3

2 entries

0.29

[(1->4)-alpha-D-galacturonide]4

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

0.24

[(1->4)-alpha-D-galacturonide]5

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

0.11

[(1->4)-alpha-D-galacturonide]6

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

0.07

[(1->4)-alpha-D-galacturonide]7

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

0.06

[(1->4)-alpha-D-galacturonide]8

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

additional information

additional information

11 entries

-

0.34

(1,4-alpha-D-galacturonide)2

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.86

(1,4-alpha-D-galacturonide)2

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

1.44

(1,4-alpha-D-galacturonide)2

Aspergillus tubingensis

-

-

136796

2.2 - 2.8

(1,4-alpha-D-galacturonide)2

Daucus carota

-

-

136786

2.24

(1,4-alpha-D-galacturonide)2

Butyrivibrio fibrisolvens

-

-

136778

0.19

(1,4-alpha-D-galacturonide)3

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

0.34

(1,4-alpha-D-galacturonide)3

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.63 - 0.97

(1,4-alpha-D-galacturonide)3

Daucus carota

-

-

136786

0.68

(1,4-alpha-D-galacturonide)3

Aspergillus tubingensis

-

-

136796

0.94

(1,4-alpha-D-galacturonide)3

Aspergillus tubingensis

-

-

136796

0.1

(1,4-alpha-D-galacturonide)4

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

0.29

(1,4-alpha-D-galacturonide)4

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.077

(1,4-alpha-D-galacturonide)5

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

0.24

(1,4-alpha-D-galacturonide)5

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.53

(1,4-alpha-D-galacturonide)5

Aspergillus tubingensis

-

-

136796

0.057

(1,4-alpha-D-galacturonide)6

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

0.11

(1,4-alpha-D-galacturonide)6

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.44

(1,4-alpha-D-galacturonide)6

Aspergillus tubingensis

-

-

136796

0.029

(1,4-alpha-D-galacturonide)7

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

0.07

(1,4-alpha-D-galacturonide)7

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.32

(1,4-alpha-D-galacturonide)7

Aspergillus tubingensis

-

-

136796

0.02

(1,4-alpha-D-galacturonide)8

Daucus carota

-

0.1 M acetate buffer pH 3.8, 30°C

671022

0.06

(1,4-alpha-D-galacturonide)8

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.074 - 0.159

(1,4-alpha-D-galacturonide)8

Daucus carota

-

heptagalacturonate

136786

0.012

(1,4-alpha-D-galacturonide)n

Malus domestica

-

-

136783

0.032

(1,4-alpha-D-galacturonide)n

Marchantia polymorpha

-

-

136774

0.06

(1,4-alpha-D-galacturonide)n

Thermotoga maritima

-

30 mM phosphate buffer pH 6.5, 80°C

668557

0.43

(1,4-alpha-D-galacturonide)n

Daucus carota

-

-

136774

3.1

(1,4-alpha-D-galacturonide)n

Aspergillus tubingensis

-

recombinant enzyme

136796

3.2

(1,4-alpha-D-galacturonide)n

Aspergillus tubingensis

-

wild type enzyme

136796

6

(1,4-alpha-D-galacturonide)n

Fusarium oxysporum

-

-

136790

0.012

digalacturonic acid

Rhodothermus marinus

D0MEP1

at pH 7.0 and 25°C

749629

0.0193

digalacturonic acid

Thermotoga thermophilus

-

at pH 6.0 and 40°C

751540

0.012 - 0.015

pectic acid

Daucus carota

-

-

136786

0.12

pectic acid

Aspergillus niger

-

enzyme form PGII

136793

0.24

pectic acid

Aspergillus niger

-

enzyme form PGI

136793

0.423

pectic acid

Daucus carota

-

-

136784

0.64

pectic acid

Fusarium oxysporum

-

-

136794

0.9

pectic acid

Geotrichum lactis

-

-

136791, 136792

0.000673

polygalacturonic acid

Rhodothermus marinus

D0MEP1

at pH 7.0 and 25°C

749629

0.00877

polygalacturonic acid

Thermotoga thermophilus

-

at pH 6.0 and 40°C

751540

0.00241

trigalacturonic acid

Rhodothermus marinus

D0MEP1

at pH 7.0 and 25°C

749629

0.0123

trigalacturonic acid

Thermotoga thermophilus

-

at pH 6.0 and 40°C

751540

0.016

[(1->4)-alpha-D-galacturonide]2

Rhizopus arrhizus

Q5W9U0

isozyme RPG16, pH 4.0, 30°C

731158

0.055

[(1->4)-alpha-D-galacturonide]2

Rhizopus arrhizus

Q5W9U0

isozyme RPG15, pH 4.0, 30°C

731158

0.34

[(1->4)-alpha-D-galacturonide]2

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

0.0092

[(1->4)-alpha-D-galacturonide]3

Rhizopus arrhizus

Q5W9U0

isozyme RPG15, pH 4.0, 30°C

731158

0.34

[(1->4)-alpha-D-galacturonide]3

Thermotoga maritima

Q9WYR8

pH 6.5, 80°C

747730

additional information

additional information

Paecilomyces variotii

-

kinetics

707200

-

additional information

additional information

Trametes sanguinea

-

kinetics

710031

-

additional information

additional information

Aspergillus giganteus

-

kinetics, overview

709377

-

additional information

additional information

Penicillium viridicatum

-

the apparent Km-value with apple pectin as substrate is 1.82 mg/ml

682497

-

additional information

additional information

Penicillium viridicatum

-

kinetics in presence and absence of Ca2+, overview

708787

-

additional information

additional information

Aspergillus niger

-

0.083 mg/ml with polygalacturonic acid at pH 4.8, 45°C

730876

-

additional information

additional information

Thermoascus aurantiacus

-

1.58 mg/ml with [(1->4)-alpha-D-galacturonide]n at pH 5.5, 60°C

729662

-

additional information

additional information

Penicillium oxalicum

-

1.79 mg/ml with [(1->4)-alpha-D-galacturonide]n for recombinant wild-type full-length enzyme, and 1.26 mg/ml with [(1->4)-alpha-D-galacturonide]n for recombinant catalytic polygalacturonase domain, pH 5.0, 50°C

729080

-

additional information

additional information

Rhizopus arrhizus

-

2.7 mg/ml with citrus polygalacturonic acid, pH 5.0, 50°C

731416

-

additional information

additional information

Aspergillus niveus

-

6.7 mg/ml with [(1->4)-alpha-D-galacturonide]n, pH 4.0, 50°C

731836

-

additional information

additional information

Rhizopus arrhizus

Q5W9U0

thermodynamic parameters of a series of galacturonic acid oligomers binding to isozyme RPG15, overview

731158

-