Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
[(1->4)-alpha-D-galacturonide]8 | pH 6.5, 80°C | Thermotoga maritima | |
0.06 | - |
[(1->4)-alpha-D-galacturonide]170 | pH 6.5, 80°C | Thermotoga maritima | |
0.07 | - |
[(1->4)-alpha-D-galacturonide]7 | pH 6.5, 80°C | Thermotoga maritima | |
0.11 | - |
[(1->4)-alpha-D-galacturonide]6 | pH 6.5, 80°C | Thermotoga maritima | |
0.24 | - |
[(1->4)-alpha-D-galacturonide]5 | pH 6.5, 80°C | Thermotoga maritima | |
0.29 | - |
[(1->4)-alpha-D-galacturonide]4 | pH 6.5, 80°C | Thermotoga maritima | |
0.34 | - |
[(1->4)-alpha-D-galacturonide]2 | pH 6.5, 80°C | Thermotoga maritima | |
0.34 | - |
[(1->4)-alpha-D-galacturonide]3 | pH 6.5, 80°C | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Thermotoga maritima | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
212000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYR8 | - |
- |
Thermotoga maritima DSM 3109 | Q9WYR8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows low activity with xylogalacturonan | Thermotoga maritima | ? | - |
? | |
additional information | the enzyme shows low activity with xylogalacturonan | Thermotoga maritima DSM 3109 | ? | - |
? | |
[(1->4)-alpha-D-galacturonide]170 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]169 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]170 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima DSM 3109 | [(1->4)-alpha-D-galacturonide]169 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]2 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | 2 D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]2 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima DSM 3109 | 2 D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]3 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]2 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]3 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima DSM 3109 | [(1->4)-alpha-D-galacturonide]2 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]4 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]3 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]5 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]4 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]6 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]5 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]7 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]6 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]8 + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]7 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA | Thermotoga maritima DSM 3109 | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
exopolygalacturonase B | - |
Thermotoga maritima |
PelB | - |
Thermotoga maritima |
TM0437 | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
73 | 95 | 73°C: 25% of maximal activity, 95°C: about 75% of maximal activity | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
105 | - |
melting temperature | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
182 | - |
[(1->4)-alpha-D-galacturonide]2 | pH 6.5, 80°C | Thermotoga maritima | |
685 | - |
[(1->4)-alpha-D-galacturonide]3 | pH 6.5, 80°C | Thermotoga maritima | |
821 | - |
[(1->4)-alpha-D-galacturonide]6 | pH 6.5, 80°C | Thermotoga maritima | |
829 | - |
[(1->4)-alpha-D-galacturonide]4 | pH 6.5, 80°C | Thermotoga maritima | |
843 | - |
[(1->4)-alpha-D-galacturonide]8 | pH 6.5, 80°C | Thermotoga maritima | |
860 | - |
[(1->4)-alpha-D-galacturonide]7 | pH 6.5, 80°C | Thermotoga maritima | |
934 | - |
[(1->4)-alpha-D-galacturonide]5 | pH 6.5, 80°C | Thermotoga maritima | |
936 | - |
[(1->4)-alpha-D-galacturonide]170 | pH 6.5, 80°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6.9 | pH 5.9: about 40% of maximal activity, pH 6.9: about 75% of maximal activity | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
534 | - |
[(1->4)-alpha-D-galacturonide]2 | pH 6.5, 80°C | Thermotoga maritima | |
2016 | - |
[(1->4)-alpha-D-galacturonide]3 | pH 6.5, 80°C | Thermotoga maritima | |
2859 | - |
[(1->4)-alpha-D-galacturonide]4 | pH 6.5, 80°C | Thermotoga maritima | |
3892 | - |
[(1->4)-alpha-D-galacturonide]5 | pH 6.5, 80°C | Thermotoga maritima | |
7461 | - |
[(1->4)-alpha-D-galacturonide]6 | pH 6.5, 80°C | Thermotoga maritima | |
12288 | - |
[(1->4)-alpha-D-galacturonide]7 | pH 6.5, 80°C | Thermotoga maritima | |
14042 | - |
[(1->4)-alpha-D-galacturonide]8 | pH 6.5, 80°C | Thermotoga maritima | |
15600 | - |
[(1->4)-alpha-D-galacturonide]170 | pH 6.5, 80°C | Thermotoga maritima |