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Literature summary for 3.2.1.67 extracted from

  • Kluskens, L.; Van Alebeek, G.; Walther, J.; Voragen, A.; De Vos, W.; Van Der Oost, J.
    Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima (2005), FEBS J., 272, 5464-5473 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
[(1->4)-alpha-D-galacturonide]8 pH 6.5, 80°C Thermotoga maritima
0.06
-
[(1->4)-alpha-D-galacturonide]170 pH 6.5, 80°C Thermotoga maritima
0.07
-
[(1->4)-alpha-D-galacturonide]7 pH 6.5, 80°C Thermotoga maritima
0.11
-
[(1->4)-alpha-D-galacturonide]6 pH 6.5, 80°C Thermotoga maritima
0.24
-
[(1->4)-alpha-D-galacturonide]5 pH 6.5, 80°C Thermotoga maritima
0.29
-
[(1->4)-alpha-D-galacturonide]4 pH 6.5, 80°C Thermotoga maritima
0.34
-
[(1->4)-alpha-D-galacturonide]2 pH 6.5, 80°C Thermotoga maritima
0.34
-
[(1->4)-alpha-D-galacturonide]3 pH 6.5, 80°C Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Thermotoga maritima 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
212000
-
gel filtration Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYR8
-
-
Thermotoga maritima DSM 3109 Q9WYR8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows low activity with xylogalacturonan Thermotoga maritima ?
-
?
additional information the enzyme shows low activity with xylogalacturonan Thermotoga maritima DSM 3109 ?
-
?
[(1->4)-alpha-D-galacturonide]170 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]169 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]170 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 [(1->4)-alpha-D-galacturonide]169 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]2 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima 2 D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]2 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 2 D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]3 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]2 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]3 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 [(1->4)-alpha-D-galacturonide]2 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]4 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]3 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]5 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]4 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]6 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]5 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]7 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]6 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]8 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]7 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]n + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]n + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
exopolygalacturonase B
-
Thermotoga maritima
PelB
-
Thermotoga maritima
TM0437
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
73 95 73°C: 25% of maximal activity, 95°C: about 75% of maximal activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
105
-
melting temperature Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
182
-
[(1->4)-alpha-D-galacturonide]2 pH 6.5, 80°C Thermotoga maritima
685
-
[(1->4)-alpha-D-galacturonide]3 pH 6.5, 80°C Thermotoga maritima
821
-
[(1->4)-alpha-D-galacturonide]6 pH 6.5, 80°C Thermotoga maritima
829
-
[(1->4)-alpha-D-galacturonide]4 pH 6.5, 80°C Thermotoga maritima
843
-
[(1->4)-alpha-D-galacturonide]8 pH 6.5, 80°C Thermotoga maritima
860
-
[(1->4)-alpha-D-galacturonide]7 pH 6.5, 80°C Thermotoga maritima
934
-
[(1->4)-alpha-D-galacturonide]5 pH 6.5, 80°C Thermotoga maritima
936
-
[(1->4)-alpha-D-galacturonide]170 pH 6.5, 80°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
5.9 6.9 pH 5.9: about 40% of maximal activity, pH 6.9: about 75% of maximal activity Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
534
-
[(1->4)-alpha-D-galacturonide]2 pH 6.5, 80°C Thermotoga maritima
2016
-
[(1->4)-alpha-D-galacturonide]3 pH 6.5, 80°C Thermotoga maritima
2859
-
[(1->4)-alpha-D-galacturonide]4 pH 6.5, 80°C Thermotoga maritima
3892
-
[(1->4)-alpha-D-galacturonide]5 pH 6.5, 80°C Thermotoga maritima
7461
-
[(1->4)-alpha-D-galacturonide]6 pH 6.5, 80°C Thermotoga maritima
12288
-
[(1->4)-alpha-D-galacturonide]7 pH 6.5, 80°C Thermotoga maritima
14042
-
[(1->4)-alpha-D-galacturonide]8 pH 6.5, 80°C Thermotoga maritima
15600
-
[(1->4)-alpha-D-galacturonide]170 pH 6.5, 80°C Thermotoga maritima