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Literature summary for 3.2.1.67 extracted from

  • Evangelista, D.E.; de Araujo, E.A.; Neto, M.O.; Kadowaki, M.A.S.; Polikarpov, I.
    Biochemical characterization and low-resolution SAXS structure of an exo-polygalacturonase from Bacillus licheniformis (2018), New Biotechnol., 40, 268-274 .
    View publication on PubMed
Search for more literature for 3.2.1.67

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 89.7% residual activity at 4 mM Bacillus licheniformis
Ca2+ 74.7% residual activity at 4 mM Bacillus licheniformis
Co2+ 36% residual activity at 4 mM Bacillus licheniformis
Cu2+ 30.4% residual activity at 4 mM Bacillus licheniformis
dithiothreitol 83.3% residual activity at 4 mM Bacillus licheniformis
Fe2+ 11.76% residual activity at 4 mM Bacillus licheniformis
Fe3+ 64.6% residual activity at 4 mM Bacillus licheniformis
K+ 96.2% residual activity at 4 mM Bacillus licheniformis
Li+ 88% residual activity at 4 mM Bacillus licheniformis
Mg2+ 91.9% residual activity at 4 mM Bacillus licheniformis
Mn2+ 68.5% residual activity at 4 mM Bacillus licheniformis
Ni2+ 73.5% residual activity at 4 mM Bacillus licheniformis
SDS 24.2% residual activity at 0.1% (w/v) Bacillus licheniformis
Triton X-100 96.6% residual activity at 0.1% (v/v) Bacillus licheniformis
Tween 20 87.4% residual activity at 0.1% (v/v) Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and Superdex 200 gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apple pectin + H2O 2% activity compared to polygalacturonic acid Bacillus licheniformis galacturonic acid
-
 ?
methylated pectin + H2O 20% activity compared to polygalacturonic acid Bacillus licheniformis galacturonic acid
-
 ?
additional information no activity with carboxymethyl cellulose and xylan Bacillus licheniformis ?
-
 ?
polygalacturonic acid + H2O 100% activity Bacillus licheniformis galacturonic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Bacillus licheniformis
monomer 1 * 48140, calculated from amino acid sequence Bacillus licheniformis

Synonyms

Synonyms Comment Organism
exo-polygalacturonanase
-
 Bacillus licheniformis
exoPG
-
 Bacillus licheniformis
pectinase
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 the enzyme maintains 65% of the maximum activity at 50°C and about 30% at 70°C Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme maintains residual activity of greater than 80% at 50°C after 15 h of incubation and after 24 h its activity is over 60% of its maximum activity Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.58
-
 polygalacturonic acid at pH 6.5 and 60°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 after incubation of the enzyme for 30 min at pH ranging from 5.0 to 10.0, no significant loss of the original enzyme activity is observed Bacillus licheniformis