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3.2.1.67: galacturonan 1,4-alpha-galacturonidase

This is an abbreviated version!
For detailed information about galacturonan 1,4-alpha-galacturonidase, go to the full flat file.

Word Map on EC 3.2.1.67

Reaction

[(1->4)-alpha-D-galacturonide]n
+
H2O
=
[(1->4)-alpha-D-galacturonide]n-1
+
D-galacturonate

Synonyms

alkaline exo-polygalacturonase, carrot exopolygalacturonase, CbPelA, corn pollen polygalacturonase, EPG, exo polygalacturonase, exo-D-galacturonanase, exo-D-galacturonase, Exo-PG, exo-PGase, exo-PGI, exo-PGII, exo-PGU B, exo-poly alpha galactouronosidase, exo-polygalacturonanase, exo-polygalacturonase, exolytic PGase, exoPG, exoPG1, exoPG2, exoPGase, exopoly-D-galacturonase, exopolygalacturonanase, exopolygalacturonase, exopolygalacturonase B, exopolygalacturonase X, Galacturan 1,4-alpha-galacturonidase, GH28, macerating enzyme, More, MpPG1, MpPG3, oligogalacturonate hydrolase, peach exopolygalacturonase, PECI, pectinase, PelB, PG III, PGase, PGase I, PGC2, PGC3, PGI, poly 1,4-alpha-D-galacturonide-galacturonohydrolase, Poly(1,4-alpha-D-galacturonide)galacturonohydrolase, poly(galacturonate)hydrolase, poly-(1-4)-alpha-D-galactosiduronate glycanohydrolase, polygalacturonanase, polygalacturonase, polymethylgalacturonase, RPG15, RPG16, SSXPG1, SSXPG2, TM0437, TmGalU, YeGH2

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.67 galacturonan 1,4-alpha-galacturonidase

Specific Activity

Specific Activity on EC 3.2.1.67 - galacturonan 1,4-alpha-galacturonidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.44
-
2 several forms of exo-D-galacturonanase
0.56
-
2 several forms of exo-D-galacturonanase
0.6
-
pH 4.5, 30°C
0.69
-
pentagalacturonic acid
1.44
-
digalacturonic acid
1.7
-
sodium pectate
1.9
-
sodium pectate
12860
-
purified enzyme, at pH 5.5, 37°C
13.5
-
exo-PG1, pH 4.0, 40°C
16
-
-
171
-
pH 5.0, 60°C
2.35
-
crude extract, at pH 6.0 and 30°C
246
-
after 9.1fold purification, at pH 4.5 and 40°C
26.99
-
crude enzyme, at pH 4.5 and 40°C
298.5
-
purified native enzyme
3.8
-
enzyme form PGII
31.4
-
exo-PG2, pH 4.0, 40°C
34.7
-
cell-free broth
346.4
-
purified native enzyme
418.6
-
12fold purified enzyme
419.4
-
purified enzyme
47
purified recombinant His-tagged enzyme, substrate [(1->4)-alpha-D-galacturonide]n, pH 6.0, 40°C
5.36
-
crude enzyme, at pH 5.5, 37°C
5.45
-
digalacturonic acid
5352
-
pH 5.5, 60°C
54
-
pH 6.8, 30°C
54.3
-
purified enzyme, pH 4.8, 45°C, substrate polygalacturonic acid
60.5
-
with citrus polygalacturonic acid, pH 5.0, 50°C
7.5
-
enzyme form PGI
98.49
-
after 42.91fold purification, at pH 6.0 and 30°C
additional information
-
with pectin as substrate: wild-type strain 97.8 U/ml, mutant strain M3 105.0 U/ml, mutant strain GH56 105.2 U/ml, and mutant strain Guserbiot 2.230 95.1 U/ml. Mutant strain M3 yields of enzyme activity is 1111.7 U/g on sugar beet and 142.1 U/g on apple pomace, while the wild-type strain gives 718.1 U/g and 240.0 U/g for sugar beet and apple pomace, respectively. Comprisons with commercial pectinases, overview