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Literature summary for 3.2.1.67 extracted from
- Expression and characterization of hyperthermostable exopolygalacturonase RmGH28 from Rhodothermus marinus (2017), Appl. Biochem. Biotechnol., 183, 1503-1515 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rhodothermus marinus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| digalacturonic acid | substrate inhibition | Rhodothermus marinus |  |
| galacturonic acid | product inhibition | Rhodothermus marinus | |
| polygalacturonic acid | substrate inhibition | Rhodothermus marinus | |
| trigalacturonic acid | substrate inhibition | Rhodothermus marinus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000673 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 0.00241 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 0.012 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin + H2O | Rhodothermus marinus | - |
galacturonic acid | - |
? | |
| citrus pectin + H2O | Rhodothermus marinus ATCC 43812 | - |
galacturonic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodothermus marinus | D0MEP1 | - |
- |
| Rhodothermus marinus ATCC 43812 | D0MEP1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Rhodothermus marinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin + H2O | - |
Rhodothermus marinus | galacturonic acid | - |
? | |
| citrus pectin + H2O | - |
Rhodothermus marinus ATCC 43812 | galacturonic acid | - |
? | |
| digalacturonic acid + H2O | - |
Rhodothermus marinus | 2 galacturonic acid | - |
? | |
| digalacturonic acid + H2O | - |
Rhodothermus marinus ATCC 43812 | 2 galacturonic acid | - |
? | |
| polygalacturonic acid + H2O | - |
Rhodothermus marinus | galacturonic acid | - |
? | |
| polygalacturonic acid + H2O | - |
Rhodothermus marinus ATCC 43812 | galacturonic acid | - |
? | |
| trigalacturonic acid + H2O | - |
Rhodothermus marinus | digalacturonic acid + galacturonic acid | - |
? | |
| trigalacturonic acid + H2O | - |
Rhodothermus marinus ATCC 43812 | digalacturonic acid + galacturonic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exopolygalacturonanase | - |
Rhodothermus marinus |
| GH28 | - |
Rhodothermus marinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 93.9 | - |
50% of the initial enzyme activity remains after 1 h incubation at 93.9°C | Rhodothermus marinus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.02 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 11.7 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 21.9 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rhodothermus marinus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000128 | - |
galacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 0.071 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 0.074 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 0.137 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 968 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 8950 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
| 9080 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
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