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Literature summary for 3.2.1.67 extracted from
- Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus (2016), Mol. Biotechnol., 58, 509-519 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) CodonPlus cells | Thermotoga thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| digalacturonic acid | - |
Thermotoga thermophilus |  |
| galacturonic acid | - |
Thermotoga thermophilus | |
| additional information | no substrate inhibition for polygalacturonate | Thermotoga thermophilus | |
| trigalacturonic acid | - |
Thermotoga thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00877 | - |
polygalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 0.0123 | - |
trigalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 0.0193 | - |
digalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Thermotoga thermophilus | - |
galacturonic acid + ? | - |
? | |
| pectin + H2O | Thermotoga thermophilus DSM 5069 | - |
galacturonic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga thermophilus | - |
- |
- |
| Thermotoga thermophilus DSM 5069 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography and Superdex 200 gel filtration | Thermotoga thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digalacturonic acid + H2O | - |
Thermotoga thermophilus | 2 galacturonic acid | - |
? | |
| digalacturonic acid + H2O | - |
Thermotoga thermophilus DSM 5069 | 2 galacturonic acid | - |
? | |
| pectin + H2O | - |
Thermotoga thermophilus | galacturonic acid + ? | - |
? | |
| pectin + H2O | - |
Thermotoga thermophilus DSM 5069 | galacturonic acid + ? | - |
? | |
| polygalacturonic acid + H2O | - |
Thermotoga thermophilus | galacturonic acid | - |
? | |
| polygalacturonic acid + H2O | - |
Thermotoga thermophilus DSM 5069 | galacturonic acid | - |
? | |
| trigalacturonic acid + H2O | - |
Thermotoga thermophilus | digalacturonic acid + galacturonic acid | - |
? | |
| trigalacturonic acid + H2O | - |
Thermotoga thermophilus DSM 5069 | digalacturonic acid + galacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 117010, calculated from amino acid sequence | Thermotoga thermophilus |
| homodimer | 2 * 121510, SDS-PAGE | Thermotoga thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-polygalacturonanase | - |
Thermotoga thermophilus |
| GH28 | - |
Thermotoga thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 86.3 | - |
the enzyme is hyperthermostable with 50% of activity remaining at 86.3°C after 1 h incubation | Thermotoga thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.55 | - |
trigalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 10.6 | - |
polygalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 14.1 | - |
digalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0079 | - |
galacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 3.63 | - |
digalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 17.2 | - |
trigalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 731 | - |
digalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 776 | - |
trigalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
| 1209 | - |
polygalacturonic acid | at pH 6.0 and 40°C | Thermotoga thermophilus | |
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